大肠癌端粒酶活性与c-myc基因表达的关系

目的　探讨端粒酶活性和c myc表达的相关性及其与大肠癌发生发展的关系。方法　运用端粒重复扩增 (TRAP)及免疫组织化学方法对 30例大肠癌、癌旁组织及正常粘膜和 2 0例大肠腺瘤性息肉组织中端粒酶活性和c myc蛋白进行检测。结果　大肠癌端粒酶活性和c myc蛋白表达率分别为 83 33% (2 5 / 30 )和 80 0 0 % (2 4 / 30 ) ,显著高于癌旁、正常大肠粘膜及大肠腺瘤性息肉组织 (P <0 0 5 ) ;端粒酶活性及c myc蛋白阳性表达率在伴淋巴结转移的大肠癌组织中明显高于无淋巴结转移者 (P <0 0 5 ) ;在 2 5例端粒酶活性阳性的大肠癌组织中有 2 3例 (92 0 0 % )表达c myc蛋白 ,端粒酶活性与c myc蛋白表达密切相关 (P <0 0 5 )。结论　端粒酶激活及c myc的异常表达与大肠癌的发生发展密切相关 ,联合检测有助于大肠癌的诊断和预后判断 ;在大肠癌的形成和发展过程中 ,c myc过度表达对端粒酶的激活可能起重要作用。

Relationship between telomerase activity and c-myc in colorectal carcinoma

Objective To explore relationship between telomerase activity and the expression of c-myc in colorectal carcinoma,and to study the occurrence and development of colorectal carcinoma.Methods Telomerase activity and expression of c-myc was measured by TRAP and immunohistochemistry.Results Telomerase activity and the expression of c-myc were 83.33% (25/30) and 80.00% (24/30) respectively in colorectal carcinoma and obviously higher than that in others tissues(P<0.05).The positive rate of telomerase activity and c-myc showed higher frequency in colorectal carcinoma with lymphatic matastasis,compared to those without lymphatic matastasis.The expression of c-myc was found to be correlated with telomerase activity in colorectal carcinoma.Conclusions Telomerase activity and the abnormal expression of c-myc are closely correlated to the generation and evolution of colorectal carcinoma,and detecting both will be helpful for the clinical diagnosis and predicting prognosis of colorectal carcinoma.The overexpression of c-myc protein may play a key role on telomerase activition during the process of carcinogenesis and progression of colorectal carcinoma.