贵州产天冬总皂苷清除氧自由基能力的研究

目的 提取贵州产天冬中总皂苷,并观察其清除氧自由基作用. 方法 提取贵州产天冬总皂苷,选用菝葜皂苷元为对照品,采用分光光度法,高氯酸显色,310 nm波长处测定其吸光度,计算天冬中总皂苷的含量;采用连苯三酚法及邻二氮菲-Fe(Ⅱ)/H₂O₂体系,用分光光度法测定天冬总皂苷对O^2-和&#8226;OH的影响. 结果以菝葜皂苷元为对照品,贵州产天冬中天冬总皂苷的含量为2.390%,RSD＝1.19%(含量均以干燥品计算);提取的天冬总皂苷在体外对O^2-具有清除作用(P<0.01),其对O^2-清除率及O2-的抑制率与天冬总皂苷含量成正相关;贵州产天冬中天冬总皂苷对&#8226;OH也有一定的清除作用,清除率为(29.1±0.17)%(P<0.05). 结论 贵州产天冬中天冬总皂苷对O^2-具有显著的清除及抑制作用,对&#8226;OH也有一定的清除作用.

Scavenging Action of Total Saponin in Asparagus Racemosus from Guizhou on Oxygen Free Radicals

Objective To detect the content of total saponin(ASP) in Asparagus racemosus from Guizhou and study its scavenging action on oxygen free radicals.Methods The total saponin was isolated from Cochinchinese Asparagus root from Guizhou.The content of which was calculated by determining absorbability with spectrophotometric method at 310 nm and coloring the perehloric acid by referring the bamboo brier sapogenin as control.The effect of ASP upon O2-and·OH was detected by the auto-oxidation in pyrogallol and phenathroline-Fe(Ⅱ)/H2O2 system.Results The content o ASP was 2.390%,RSD=1.19%.ASP cleared O2-in vitro(P<0.01) in a dose-dependant manner.It inhibited the generation of ·OH in vitro by(29.1±0.17)%(P<0.05).Conclusion ASP in Asparagus racemosus from Guizhou could scavenge O2-markedly and inhibit ·OH as we11.