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| 外生型肝癌细胞系(EGHC-9901)的建立 | |
| 引用本文： | 吴小鹏,王占民,刘博,刘军,高英茂,李兆亭,刘春生. 外生型肝癌细胞系(EGHC-9901)的建立[J]. 中国现代普通外科进展, 2000, 3(2): 23-25 |
| 作者姓名： | 吴小鹏 王占民 刘博 刘军 高英茂 李兆亭 刘春生 |
| 作者单位： | 1. 山东医科大学附属医院普外科,山东,济南,250012 2. 山东医科大学组织胚胎学教研室,山东,济南,250012 3. 山东医科大学附属医院肿瘤中心,山东,济南,250012 |
| 摘 要： | 目的 介绍外生型肝癌细胞系的建立及对其生物学特性测定的结果。方法 将手术切除的肿瘤标本进行体外培养，观察肝癌细胞形态学改变，进行核型分析、增殖动力学、流式细胞计数、裸鼠成瘤情况及AFP测定。结果 外生型肝癌细胞系(EGHC-9901)无论形态学观察，还是功能学测定，均符合肝癌细胞的一般特征，并具有体外分泌AFP功能。肝癌细胞之间形成完整毛细胆管，内有明显微绒毛。结论 EGHC-9901细胞系符合外生型肝癌细胞系的特征。 |
| 关 键 词： | 外生型肝癌细胞系 肝细胞 肿瘤细胞 细胞培养 EGHC-9901细胞系 |
| 修稿时间： | 2000-06-01 |
ESTABLISHMENT OF HUMAN EXTRAHEPATIC GROWING HEPATOCELLULAR CARCINOMA CELL LINE EGHC-9901 |
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| WU Xiao-peng,WANG Zhan-min,LIU Bo,LIU Jun,GAO Ying-mao,LI Zhao-ting,LIU Chun-shenDepartement of General Surgery,The Affiliated Hospital of Shandong Medical Vnivercity. ESTABLISHMENT OF HUMAN EXTRAHEPATIC GROWING HEPATOCELLULAR CARCINOMA CELL LINE EGHC-9901[J]. Chinese Journal of Current Advances in General Surgery, 2000, 3(2): 23-25 | |
| Authors: | WU Xiao-peng WANG Zhan-min LIU Bo LIU Jun GAO Ying-mao LI Zhao-ting LIU Chun-shenDepartement of General Surgery The Affiliated Hospital of Shandong Medical Vnivercity |
| Abstract: | To establish a new extrahepatic growing hepatocellular carclnoma cell line. Methods: A speciment that was pathologically identified extrahepatic growing hepatocellular carcinoma were cultured in vitro. The morphology,karyotype analysis, DNA analysis, the tumor formation of heterotransplantation were observed. Results: Morphological observation and functional analysis showed that it had the comnnon features of HOC.It was found to be able to secret AFP in vitro. Conclusion: EGHC-9901 has characteristic of the extrahepatic growing hepatocellular carcinoma cell line. |
| Keywords: | Carclnoma hepatocellular Tumer cells culeured |
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