麻风杆菌特异酚糖脂血清学活性的初步研究

作者对麻风杆菌特异性抗原PG1的血清学活性并与其终端糖的人工合成抗原BGG进行比较研究,指出PG1-ELISA与BGG-ELISA显著相关,高度敏感特异。比较了ML-ELISA.PG_1ELISA与FLA-ABS.T三法的检测结果显著相关。提示三法均是麻风免疫流行病学研究的有用工具。但因BGG能人工合成,将是人们最寄希望的方法。提出用EA代替BSA与GS封闭非特异性反应、简便、价廉、效果好,值得推广。

Preliminary Seudy on Serological Activity ot Phenolic Glycolipid and Its Application of Leprosy

In this article, we conducted:1. Study on comparisons of serological activity between phenolic glycolipid (PGI) and its terminal sugar. The sera for comparison were collected from leprosy patients (182), tuberculosis patients (20), and normal persons (108) in non-endemic area. The results indicated highly significant positive correlation (rBGG = 0.9953 P<0.0005) between them, the MOD values in sera from normal persons being similar to that of sera from tuberculosis patients. These suggested that PG1-ELISA and BGG-ELISA are highly specific for detection of infection with M. leprae. Comparing PG1-ELISA with ML-ELISA, the sensitivity of both tests to sera from leprosy patients was found to be the same, suggesting of PG1-ELISA and BGG-ELISA being as sensitive as ML-ELISA.Thus, PG1-or BGG-ELISA is highly sensitive and specific for detection of infection with M. leprae.2. Study on correlation PG1-ELISA with ML-ELISA and FLA-ABS.T. The results indicated that they are also highly significant positive correlation (rFLA-ABS.T = 0.945 P<0.01; rML-ELISA = 0.972 p<0.005).3. Study on blocking non-specific binding. The results indicated that conventional BSA and GS can be replaced with EA. The efficiency of EA is not only equal to BSA and GS, but also facile, cheaper and easier to get. The authors suggest application for blocking non-specific binding to ML-ELISA and PG1-ELISA or BGG-ELISA.