| 人乳腺癌原代细胞和细胞系中肿瘤干细胞的比较 |
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| 引用本文： | 李治,刘春萍,贺艳丽,田元,黄韬.人乳腺癌原代细胞和细胞系中肿瘤干细胞的比较[J].中国医科大学学报,2008,37(2):155-157. |
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| 作者姓名： | 李治 刘春萍 贺艳丽 田元 黄韬 |
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| 作者单位： | 华中科技大学,协和医院乳腺甲状腺外科,武汉,430022 |
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| 基金项目： | 国家自然科学基金
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湖北省科技攻关项目 |
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| 摘 要： | 目的比较人乳腺癌原代细胞以及人乳腺癌细胞系MCF-7、MDA-MB-231S中乳腺癌干细胞的含量和细胞表型。方法采用流式细胞技术对人乳腺癌原代细胞以及MCF-7、MDA-MB-231S进行检测和分选,并测定不同细胞亚群在裸鼠体内重建肿瘤的能力。结果人乳腺癌原代细胞以及MCF-7、MDA-MB-231S细胞的CD44和CD24表达与分布不同;和人乳腺癌原代细胞相比,MCF-7、MDA-MB-231S细胞中乳腺癌干细胞的含量更高(P<0.01)。结论和人乳腺癌原代细胞相比,人乳腺癌细胞系中含有更高比例的乳腺癌干细胞,并且细胞表型也发生了改变。
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| 关 键 词： | 干细胞 原代细胞 乳腺癌 细胞表型 |
| 文章编号： | 0258-4646(2008)02-0155-03 |
| 修稿时间： | 2007年7月6日 |
Compare Cancer Stem Cells in the Human Breast Cancer Cell Line with Those in the Primary Cells |
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| LI Zhi,LIU Chun-ping,HE Yan-li,TIAN Yuan,HUANG Tao.Compare Cancer Stem Cells in the Human Breast Cancer Cell Line with Those in the Primary Cells[J].Journal of China Medical University,2008,37(2):155-157. |
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| Authors: | LI Zhi LIU Chun-ping HE Yan-li TIAN Yuan HUANG Tao |
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| Abstract: | Objective To compare the content and phenotype of cancer stem cells in the MCF-7,MDA-MB-231S human breast cancer cell line with those in the human breast cancer primary cells.Methods Different subpopulation cells were detected and separated from MCF-7,MDA-MB-231S and human breast cancer primary cells by flow cytometry,and their ability to reconstruct tumor was determined in vivo.Results The expression and distribution of CD44 and CD24 in MCF-7,MDA-MB-231S and human breast cancer primary cells were different.Compared with human breast cancer primary cells,the proportion of cancer stem cells in MCF-7 and MDA-MB-231S cells was higher(P < 0.01).Conclusion Compared with human breast cancer primary cells,there are more breast cancer stem cells in the human breast cancer cell line and the phenotype has been changed. |
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| Keywords: | stem cell primary cell breast cancer cell phenotype |
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