异基因小鼠胚胎胸腺移植治疗系统性红斑狼疮模型鼠的实验观察

目的探讨胚胎胸腺组织移植对系统性红斑狼疮(SLE)模型鼠的治疗效果。方法将21只符合要求的BALB/c×C57BL/6F1小鼠经尾静脉注射亲代淋巴细胞混悬液后随机分成3(A～C)组,于SLE模型建立成功后接受相应治疗方案,第25天处死模型小鼠,采用流式细胞术检测各组小鼠外周血中CD4+/CD8+T细胞的比值;电子天平称取小鼠胸腺湿质量后计算胸腺指数;直接免疫荧光法检测肾冰冻切片免疫复合物的荧光强度;间接免疫荧光法检测血清中抗核抗体(ANA)的荧光滴度。结果①C组外周血中CD4+/CD8+T细胞的比值显著高于A组(P<0.05),但A组与B组相比,差异无显著性(P>0.05);②B组胸腺指数与A组相比,差异无显著性(P>0.05),但显著低于C组(P<0.05);③C组肾脏冰冻切片荧光强度及外周血ANA荧光滴度均低于A组和B组(P<0.05)。结论胚胎胸腺移植对SLE模型鼠有显著治疗效果,有望运用于临床SLE的治疗。

Experimental Observation of Xenogenous Fetal Thymus Transplantation on Systemic Lupus Erythematousus Mice

Objective To explore the therapeutic effects of fetal thymus transplantation on systemic lupus erythematosus(SLE) mice.Methods Twenty-one BALB/c×C57BL/6 F1 mice were divided into 3 groups after their being injected with their parental lymphocytes suspended solutions.When the models were constructed successfully,group A accepted a therapy by adrenaline;Group B accepted a therapy by fetal thymus transplantation;Group C adrenaline and fetal thymus transplantation.Twenty-five days after the therapies,mice serum were detected by flow cytometry(FCM) for T cells ratio of CD4/CD8;Thymus were weighed with an elecronic equi-armbalance,then the indexes of thymus were calculated;Direct immunofluorescence technique was used to test the intensities of fluorescence of mice kidney frozen sections;The serum fluorescence titres of anti-nuclear antibodies(ANA) were tested by indirect immunofluorescence technique.Results ①The T cells CD4/CD8 ratios in group C was higher than that in group A(P<0.05),while the ratios had not significant difference in group A and group B(P>0.05); ②Thymus indexes in group C had not significant difference compared with that of group B(P>0.05),while it was higher in group C than that in group A(P<0.05);③The fluorescence intensities of mice kidney frozen sections and ANA titres in group C were lower than that in group A and B(P<0.05).Conclusion Fetal thymus transplantation performed significant therapeutic effects in SLE mice,and it could be used in clinical treatment of SLE.