乳腺癌组织CAS蛋白的表达及其与细胞增殖和凋亡的关系

目的 分析乳腺癌组织CAS蛋白的表达水平与细胞增殖、凋亡指数及患者临床指标的关系.方法 收集普通导管增生20例、异型导管增生20例、导管原位癌10例、浸润性导管癌53例、正常乳腺组织14例的标本,用免疫组织化学方法观察标本组织中CAS蛋白的表达,用ki-67标记细胞增殖指数,用TUNEL法测定凋亡指数,并进行相关统计学分析.结果 CAS在正常乳腺、普通导管增生、异型导管增生、导管原位癌、浸润性导管癌中的阳性率逐渐升高,分别为14.3％(2/14)、25.0％(0/20)、40.0％(8/20)、60.0％(6/10)、75.5％(40/53),差异有统计学意义(x2=29.382,P=0.000);CAS的表达与乳腺浸润性导管癌患者的年龄、肿瘤大小、肿瘤分期无关,与肿瘤的组织学分级、核分裂像、淋巴结转移有关(P＜0.01,P＜0.05);CAS评分与细胞增殖指数呈正相关(r=0.439,P=0.003),而与凋亡指数无相关性(r=0.248,P=0.083).结论 乳腺癌组织中,CAS的表达和细胞增殖指数间具有一定的相关性.

CAS protein expression in invasive breast ductal carcinoma and its relationship with cell proliferation and apoptosis

Objective To analyze the relationship of CAS protein expression with proliferative index,apoptosis index and clinical parameters in breast cancer tissues.Methods Immunohistochemistry for CAS expression,ki-67(proliferative index) and TUNEL(apoptosis index) were examined in 20 usual ductal hyperplasia(UDH),20 atypical ductal hyperplasia(ADH),10 ductal carcinoma in situ(DCIS),53 invasive ductal carcinoma(IDC) and 14 normal breast tissues.Results CAS expression increased in order in the normal breast tissues,UDH,ADH,DCIS and IDC,with the positive rates of CAS protein being 14.3%(2/14),25.0%(0/20),40.0%(8/20),60.0%(6/10),and 75.5%(40/53),respectively(χ2=29.382,P=0.000).CAS protein expression was correlated with histological grade,mitotic activity,and lymph node metastasis of IDC(P<0.01,P<0.05),and not with patient age,tumor volume or grade.CAS protein expression was positively correlated with ki-67 index(r=0.439,P=0.003),and not with the apoptosis index(r=0.248,P=0.083).Conclusion CAS protein expression is associated with cell proliferation index in breast cancer tissues.