鞘内注射钙/钙调素依赖性蛋白激酶Ⅱ抑制剂对坐骨神经损伤大鼠的镇痛作用

目的 探讨钙/钙调素依赖性蛋白激酶Ⅱ(CaMKⅡ)在神经病理性疼痛中的作用.方法 大鼠48只随机分为6组:坐骨神经分支选择性损伤组(SNI组)、假手术组(Sham组)、术前鞘内注射AlP(autocamtide-2-related inhibitory peptide AIP)或生理盐水组(BA组、BN组)、术后鞘内注射AIP或生理盐水组(AA组、AN组).测定SNI大鼠注射AIP前后机械缩足阈值(mechanical withdrawal threshold,MWT)的改变.结果 SNI大鼠从术后第2天至第14天出现明显的机械痛敏,鞘内注射AIP能够缓解SNI大鼠的机械痛敏,术前给药组术后1 d、2d、3d的MWT分别为(9.0±1.1)g、(9.1±1.5)g、(7.5±1.5)g,显著高于SNI组[分别为(5.9±0.9)g、(5.6±1.0)g、(5.7±1.0)g,均P<0.01];术后给药能缓解SNI大鼠的机械痛敏约4h,较之术前给药为短.结论 CaMKⅡ参人了神经病理性痛大鼠疼痛的调节.

The effect of intrathecal administration AIP on pain behavior in rats with spared nerve injury

objective To investigate the effect of calcium/calmodulin-dependent protein kinase Ⅱ(CaMK Ⅱ)in neuropathic pain.Methods In this study,model were established spared nerve injury in rats.48 rats were divided into 6 groups randomly(n=8):rats with spared nerve injury(SNI group),sham group,intrathecal injection AIP(an inhibitor of CaMK Ⅱ)or NS before operation(AA group,ANgroup),intrathecal injection AIP or NS after operation(BA group,BN group).The changes of mean withdrawal threshold(MWT)were observed before and after injection.Results There were significant increases mechanical alloydynia in SNI rats.On day 1,2,3 after injection,compared with AN group[(9.0±1.1)g,(9.1±1.5)g,(7.5±1.5)g],AA group's MWT[(5.9±0.9)g,(5.6±1.0)g,(5.7±1.0)g]increase significantly(P＜0.01).Administration AlP postoperation can relieve the SNI rat's mechanical alloydynia for 4 hours.The analgesia effect of preemptive administration AIP in rats is longer than administration AlP postoperation.Conclusion CaMK Ⅱ may be involved in the modulation of neuronpathic pain in rats.