| HPLC法测定复方菝葜颗粒中落新妇苷的含量 |
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| 引用本文： | 陈新元,谢谊,刘玉琴,王实强.HPLC法测定复方菝葜颗粒中落新妇苷的含量[J].湖南中医杂志,2014(11):163-165. |
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| 作者姓名： | 陈新元 谢谊 刘玉琴 王实强 |
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| 作者单位： | 湖南国华制药有限公司;湖南省中医药研究院中药研究所;湖南中医药大学 |
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| 摘 要： | 目的：建立高效液相法测定复方菝葜颗粒中落新妇苷含量的测定方法。方法：采用Waters SymmetryC18（150×4.6mm,5μm）为色谱柱,流动相为甲醇-0.3%冰醋酸（32∶68）,流速为1.0m L/min;检测波长为291nm。结果：落新妇苷的线性范围为0.12~1.2μg,平均回收率为98.19%,RSD为0.82%。结论：所建立方法操作简便、准确、可靠、重复性好,可用于复方菝葜颗粒中落新妇苷的含量测定。
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| 关 键 词： | 高效液相色谱法 复方菝葜颗粒 落新妇苷 含量测定 |
Determination of astilbin in compound Baqia granules by high-performance liquid chromatography |
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| Institution: | CHEN Xin - yuan , XIE Yi, LIU Yu - qin, WANG Shi - qiang ( 1. Hunan Guohua Pharmaceutical Co. , Ltd. , Changsha 410013, Hunan, China ; 2. Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 410013, Hunan, China; 3. Hunan University of Chinese Medicine, Changsha 410208, Hunan, China) |
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| Abstract: | Objective： To establish a method for determining the content of astilbin in Baqia granules by high-performance liquid chromatography（ HPLC）. HPLC was performed on a Waters Symmetry C18column（ 150 ×4. 6mm,5μm） with a mobile phase of methanol-0. 3% glacial acetic acid（ 32∶ 68） at a flow rate of 1. 0ml / min and a detection wavelength of 291 nm. Results： A linear relationship was found within an astilbin content range of0. 12-1. 2μg. The average recovery rate was 98. 19%,and the relative standard deviation was0. 82%. Conclusion： This HPLC method is simple,accurate,reliable,and repeatable and can be used for the determination of astilbin in compound Baqia granules. |
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| Keywords: | high-performance liquid chromatography compound Baqia granule astilbin determination |
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