Association of genetic variants with anti-tuberculosis drug induced hepatotoxicity: A high resolution melting analysis

BackgroundTuberculosis (TB) treatment remains a challenge owing to the high incidence of drug induced hepatotoxicity (DIH). Apart from environmental factors, single nucleotide polymorphisms (SNPs) in drug metabolizing enzymes (DMEs), nuclear receptors (NRs) and transporter proteins (TPs) contribute to DIH. In the present study, we report known and novel SNPs in a total of seven genes of DMEs, NRs and TPs with high resolution melting (HRM) technique.MethodsDNA samples of 185 TB patients of Western Indian population, of which 50 showed DIH, were analyzed. Grouping of the temperature-shifted difference plots obtained from the DNA melt curves enables identification of known and novel SNPs. Representative samples of each group were sequenced.ResultsWe report 18 novel SNPs, of which 3 are in 5′-UTR, 14 in exonic and 1 in intronic region. Of the SNPs in exons, 7 non-synonymous, 3 synonymous and 4 deletion mutations were observed. Among the known SNPs, CYP2E1 wild-type, NAT2¹5 mutant and NAT2¹6 heterozygous genotypes were associated with DIH (p < 0.05). Among the novel SNPs, group 2 of SLCO1B1 showed a significant association (p < 0.05).ConclusionsWhile several SNPs showed borderline p values between 0.05 and 0.15, the confidence in association can be improved further by using larger data sets.