Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia |
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| Affiliation: | 1. Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia;2. Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia;3. Division of Microbiology, Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;4. Biomedical Science and Molecular Typing Laboratory, Institute of Graduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia;5. Infectious Disease Epidemiology Group, Sir Henry Wellcome Laboratories, Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, UK;6. NIHR Southampton Respiratory Biomedical Research Unit, Southampton, UK;7. Institute of Life Sciences, University of Southampton, UK;8. Public Health England, Southampton, UK;9. Specialized Diagnostic Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia;1. Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;2. Centre for Biodiscovery and Molecular Development of Therapeutics, Queensland Tropical Health Alliance, James Cook University, Cairns, Queensland 4878, Australia;3. Department of Microbiology, Immunology and Tropical Medicine, and Research Center for Neglected Tropical and Infectious Diseases of Poverty, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA;4. Tropical Disease Research Laboratory, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;5. Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;1. Department of Parasitology, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India;2. Regional Medical Research Center (ICMR), Dibrugarh 786 001, Assam, India;1. Child Health Research Foundation, Department of Microbiology, Dhaka Shishu Hospital, Dhaka 1207, Bangladesh;2. International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA;3. Department of Pediatrics, Stanford University School of Medicine, Stanford, CA, USA;4. Respiratory Diseases Branch, US Centers for Disease Control and Prevention, Atlanta, GA, USA;5. Bangladesh Institute of Child Health, Dhaka Shishu Hospital, Dhaka, Bangladesh;1. Faculty of Medicine, Infectious Disease Epidemiology Group, University of Southampton, Mailpoint 814, Level C, Sir Henry Wellcome Laboratories, South Block, University Hospital Southampton Foundation NHS Trust, Southampton SO16 6YD, UK;2. University of Southampton Malaysia Campus, No. 3, Persiaran Canselor, 1, Kota Ilmu, Educity, Iskandar, 79200 Nusajaya, Johor, Malaysia;3. Global Health Research Institute, Faculty of Medicine, University of Southampton, Southampton SO16 6YD, UK;1. University of Maryland-College Park, College Park, MD 20740, United States;2. United States Department of Agriculture, Beltsville Agricultural Research Center, Building 1040, Room 103, 10300 Baltimore Avenue, Beltsville, MD 20705-2350, United States |
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| Abstract: | Streptococcus pneumoniae is an epidemiologically important bacterial pathogen. Recently, we reported the antibiotic susceptibility patterns of a limited collection of pneumococcal isolates in Malaysia with a high prevalence of erythromycin resistant strains. In the present study, 55 of the pneumococcal isolates of serotype 19F were further analysed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The generated genotypic patterns were then correlated with the antibiograms previously reported. Forty-seven different PFGE profiles (PTs) were obtained, showing that the isolates were genetically diverse. MLST identified 16 sequence types (STs) with ST-236 being predominant (58.2%), followed by ST-81 (10.3%). Among the ST-236 isolates, 22 were erythromycin resistant S. pneumoniae (ERSP) and 15 were trimethoprim/sulfamethoxazole (TMP/SMX) resistant, while among ST-81, four isolates were ERSP and two were TMP/SMX resistant. The high prevalence of erythromycin resistant serotype 19F isolates of ST-236 in this study has also been reported in other North and South East Asian countries. |
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| Keywords: | Serotype 19F Antibiogram Multilocus sequence type Pulsed field gel electrophoresis |
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