配伍药物与pH值环境对大黄蒽醌类成分溶出变化的影响规律

目的 研究配伍药物与pH值环境对大黄药对中蒽醌类成分溶出变化的影响规律。方法 首先检测与大黄配伍的药物（醋甘遂、牡丹皮、黄芩、黄连、附子、枳实、厚朴）单煎液的pH值，然后在相同pH值的水溶液中加入大黄进行煎煮，采用UV-Vis法和HPLC法测定此pH值的水煎液中蒽醌类成分的量，与大黄药对共煎液中蒽醌类成分的量进行比较。结果 UV-Vis法检测结果显示，大黄与黄连配伍时，总蒽醌的溶出量最低，而大黄与黄芩配伍时总蒽醌的溶出量最高。用盐酸水溶液提取大黄，总蒽醌的溶出量随pH值升高而增加；HPLC法测定结果显示，在测定的7个药对中，黄连与大黄配伍时，蒽醌类成分溶出量最低，而附子与大黄配伍时，蒽醌类成分的溶出量最高。使用不同pH值的盐酸水溶液提取大黄时，蒽醌类成分的量在pH值为5.6时最高。结论 大黄在煎煮过程中，与其配伍的药物和pH值环境均会引起蒽醌类成分溶出量的变化，但是不同药物配伍后形成的pH值环境与用盐酸调整的pH值环境对蒽醌类成分产生的影响存在不一致性，pH值环境对其影响较大。

Effects of compatible herbs and pH value conditions on change rule of anthraquinones in Rhei Radix et Rhizoma

Objective To study the effects of various compatible herbs and pH value conditions on the change rule of anthraquinones in Rhei Radix et Rhizoma (RRR). Methods The pH value of the extracted solution from seven compatible herbs vinegar Kansui Radix (vKR), Moutan Cortex (MC), Scutellariae Radix (SR), Coptidis Rhizoma (CR), Aconiti Lateralis Radix Praeparata (ALRP), Aurantii Fructus Immaturus (AFI), and Magnoliae Officinalis Cortex (MOC)] were determined, then RRR was added into hydrochloric acid solution with the same pH value as the above solutions and boiled, in which the contents of anthraquinones were determined by UV-Vis and HPLC. The contents of anthraquinones were compared with those in the decoction of compatible herbs and single RRR. Results The results of UV-Vis showed that total anthraquinones got the lowest amount when RRR and CR were boiled together while the highest appeared when RRR and SR were boiled together; the contents of total anthraquinones were increased when the pH value was increased. The results of HPLC showed that the five anthraquinones got the lowest dissolving-out quantity when RRR and CR were boiled together while the highest appeared when RRR and ALRP were boiled together. Under the conditions of different pH values, the highest dissolving-out quantity was got when the pH value reached 5.6. Conclusion Both the compatible herbs and pH value could affect the dissolution of anthraquinones during the extraction. However, the effects of various compatible herbs and different pH value conditions adjusted by hydrochloric acid are different, and the pH value conditions have greater effects.