鱼藤酮对大鼠脑内5-羟色胺能神经元损伤的机制研究

目的研究鱼藤酮对大鼠脑内5-羟色胺能神经元的毒性作用及其机制。方法健康成年雄性Wistar大鼠背部皮下注射鱼藤酮制备帕金森病动物模型。采用免疫组化、蛋白印迹及分光吸光度法检测大鼠中脑中缝背核5-羟色胺能神经元的损伤及中缝背核氧化应激参数(丙二醛和还原型谷胱甘肽)的改变。结果和对照组相比,鱼藤酮组大鼠脑内中缝背核5-羟色胺(5-HT)免疫反应阳性神经元数明显少于对照组(P<0.01),Western blot结果显示中缝背核5-HT表达在鱼藤酮组明显降低(P<0.01);鱼藤酮组大鼠中缝背核区域丙二醛含量明显增高(P<0.01)、还原型谷胱甘肽(GSH)含量明显减少(P<0.01)。结论鱼藤酮在损伤大鼠脑内多巴胺能神经元的同时对5-HT能神经元也具有明显的毒性作用,氧化应激可能是导致5-HT神经元损伤的主要原因。

The damaged mechanism of rotenone on serto onergic neur ons of brain in rats

Objective To study the toxic mechanism of rotenone on serotonergic neurons of brain in rats .Methods Healthy adult male Wistar rats were injected rotenone subcutaneous to prepare for Parkinson＇s disease animal model.The damage of serotonergic neurons of the dorsal raphe nucleus in rats brain was detected using immunocytochemistry, Western blot and spectrophotometry techniques.Results The numbers of 5-HT immune response positive neuron significantly reduced in the dorsal raphe nucleus of rotenone group as compare with control group （P〈0.01）.The results of Western blot showed that the expression of 5-HT in the dorsal raphe nucleus was significantly decreased in rotenone group ras （P〈0.01）.Compared with control group, oxidative damage significantly increased in the dorsal raphe nucleus of rotenone group rats.Conclusion Rotenone bears obvious toxicity to serotonergic neurons, and oxidative stress might be the may pathogenesis of rotenone on serotonergic neurons in rats.