| 风疹病毒单克隆抗体的研究 |
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| 引用本文： | 赵丽,韩世杰,范桂香,房益兰.风疹病毒单克隆抗体的研究[J].山东大学学报(医学版),1991(4). |
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| 作者姓名： | 赵丽 韩世杰 范桂香 房益兰 |
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| 作者单位： | 山东医科大学病毒学研究室，山东医科大学病毒学研究室，西安医科大学分子病毒学研究室，西安医科大学分子病毒学研究室 |
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| 摘 要： | 用风疹病毒(RV)Gos-10株免疫BALB/c小鼠,取其脾细胞与Sp2/0细胞融合,用ELISA法筛选分泌RV McAb的杂交瘤细胞6株,经克隆培养后发现其中的1A_9株能稳定分泌高效价特异性McAb,经鉴定为IgG2a,经ELISA检测、抗原吸收试验、中和试验及HI试验征明该McAb是种特异性的,能与各株RV发生交叉反应,而与其它种病毒不发生反应,证明此McAb对RV抗原有高度特异性与敏感性,可用以制备诊断试剂,实现风疹的早期、快速诊断和基础理论研究。
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| 关 键 词： | 风疹病毒 抗体 单克隆 酶联免疫吸附测定 Sp 2/0细胞 小鼠 纯系BALBC |
THE STUDY ON MONOCLONAL ANTIBODY TO RUBELLA VIRUS |
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| Zhao Li,Han Shijie Dept.of Virology,Shandong Medical University.THE STUDY ON MONOCLONAL ANTIBODY TO RUBELLA VIRUS[J].Journal of Shandong University:Health Sciences,1991(4). |
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| Authors: | Zhao Li Han Shijie Deptof Virology Shandong Medical University |
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| Abstract: | This experiment which fused spleen cells of BALB/c mice im-munized by rubella virus Gos-10 strain with Sp2/0 myeloma cells,screened by ELISA has got six hybridomas.The 1A_9 hybridoma which can secrete RV McAb steadly,was cloned for 3 times with limited dilution method and po-sitive rate reached as high as to 100 per cent.This RV McAb was classified as sub-IgG2a.ELISA antigen absorption test,neutralization test,and haemag-glutination inhibition test showed that the McAb is specific and sensitive to RV and can be used to prepare diagnostic kits to diagnose RV infection early and rapidly. |
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| Keywords: | Rubella virus Antibodies monoclonal Enzyme linked immunosorbent assay Sp2/0 myeloma cells Mice inbred BALB C |
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