乙酰唑胺对表达水孔蛋白1的非洲爪蟾卵母细胞转运水功能的影响

目的观察乙酰唑胺对水孔蛋白1(AQP1)水转运功能的影响。方法将AQP1 cRNA 10 ng注射入非洲爪蟾卵母细胞,观察并记录给予1×10^-7～1×10^-5 mol·L^-1乙酰唑胺后卵母细胞在低渗溶液中的膨胀率,并计算其渗透水通透性(Pf)的变化。结果非洲爪蟾卵母细胞表达AQP1蛋白后,在低渗溶液中迅速膨胀,Pf值显著增加至1.82×10^-2 cm·s^-1,而注射水的阴性对照仅为0.25×10^-2 cm·s^-1。AQP抑制剂HgCl₂使Pf值降至0.64×10^-2 cm·s^-1;1×10^-7～1×10^-5 mol·L^-1乙酰唑胺处理后,Pf值分别降至1.43,1.24和0.93×10^-2 cm·s^-1。结论乙酰唑胺剂量依赖性地降低AQP1介导的渗透水通透性,抑制AQP1转运水的功能。

EFFECT OF ACETAZOLAMIDE ON THE WATER TRANSPORTING FUNCTION OF XENOPUS OOCYTES EXPRESSING AQUAPORIN-1

AIMTo observe the effect of acetazolamide on the water transporting function of Xenopus oocytes expressing aquaporin-1. METHODSFifty nL AQP1 cRNA was injected into Xenopus oocytes, then the cells were transferred to low osmotic solution. The swelling of the cells in response to 1×10^-7～1×10^-5 mol·L^-1 acetazolamide was recorded, and the corresponding osmotic water permeability (Pf) was calculated. RESULTSThe Xenopus oocytes expressing AQP1 protein swelled immediately after transferred to low osmotic solution, with the Pf markedly increased to 1.83×10^-2 cm·s^-1. While the Pf of negative group injected with ddH₂O remained as 0.25×10^-2 cm·s^-1. Aquaporin inhibitor HgCl₂ decreased the Pf of AQP1 to 0.64×10^-2 cm·s^-1; After treated with acetazolamide at the dose of 1×10^-7～1×10^-5 mol·L^-1, the Pf decreased in a concentration-dependent manner. CONCLUSIONAcetazolamide decreased the osmotic water permeability induced by AQP1 and inhibited the water transporting function of AQP1 in a dose dependent manner.