诊断用彩色多普勒超声辐照对小鼠早孕胚胎细胞超微结构的影响

目的:应用彩色多普勒超声辐照早孕小鼠胚胎,观察小鼠早孕胚胎细胞超微结构的变化。方法:8只孕6~7 d成年昆明种小鼠,按彩超辐照时间不同随机等分为4组,组(对照组)、组(辐照10 min组)、组(辐照20 min组)、组(辐照30 min组),仪器采用百胜公司DU-8型彩色超声诊断仪。照射条件:腹部凸阵探头,探头频率为3.0MHz,MI=0.9 TiS=0.9。照射后24 h取小鼠胚胎标本,HE染色光镜观察组织结构,透射电镜观察超微结构变化。结果:各组光镜下观察胚胎组织结构均未见异常。照射30 min组小鼠胚胎细胞电镜检查发现染色质浓缩边集、线粒体肿胀等超微结构变化。结论:彩色多普勒超声辐照早孕小鼠可引起胚胎细胞超微结构发生改变。

Influence of diagnostic CDFI on ultrastructure of the rats embryos cell

Objective:To study the influence of diagnostic CDFI on ultrastructure of the rats embryos cell.Methods:Eight female rats that had been pregnant for 6～7 days were divided into four groups averagely and randomly:control group(group I)was sham-irradiated;group Ⅱ-Ⅳ were irradiated by diagnostic CDFI(H=3.0 MHz,Tis=0.9,MI=0.9)for 10 min,20 min and 30 min respectively.The fetal rats'embryos of the four groups were resected after 24 hours irradiation and HE stain was done for microscopic observation;ultrastructural changes were observed by transmission electronmicro scope.Results:There was no abnormal changes among group Ⅰ-Ⅳ under microscope.The ultrastructural changes of the embryos cell in group Ⅳ were observed by transmission electronmicroscope,which showed condensation of the nuclear chromatin with margination,mitochondria swelling.etc.Conclusions:Continuous irradiation for 30 min by diagnostic CDFI on fetal rat in pregnancy may result in ultrastructural changes.