桔梗皂苷D对MG-63细胞的凋亡作用

目的 研究桔梗皂苷D对MG-63细胞的促凋亡作用.方法 MTT法测定桔梗皂苷D对MG-63细胞增殖抑制情况及其IC50值,利用流式细胞仪检测加入桔梗皂苷D作用24 h后的MG-63细胞的凋亡情况、凋亡类型.使用二氯荧光黄二乙酸酯(DCFH-DA)检测加入桔梗皂苷D后肿瘤细胞内活性氧水平,加入JC-1线粒体染料试剂盒检测被桔梗皂苷D孵育24 h后肿瘤细胞线粒体膜电位的变化.结果 MTT数据表明桔梗皂苷D对细胞的生长半数抑制浓度IC50为11.80μmol/L.MG-63细胞被桔梗皂苷D作用24 h后,通过JC-1和DCFH-DA染色的实验结果表明桔梗皂苷D作用后的MG-63细胞内的活性氧水平上升,细胞线粒体膜电位下降.结论 桔梗皂苷D可能通过增加细胞内活性氧水平破坏线粒体从而导致细胞发生凋亡.

Effect of platycodin D on the apoptosis of MG-63 cells

Objective To investigate the proapoptotic effect of platycodin D on MG-63 cells. Methods The proliferation of MG-63 cells was detected by MTT assay. The cell apoptosis and type of apoptosis were determined by flow cytometry. The levels of intracellular ROS and mitochondrial membrane potential in MG-63 cells were detected by DCFH-DA and JC-1 staining, respectively. Results The IC50 of platycodin D on MG-63 cells was 11.80μmol/L. The ROS level was increased, but the mitochondrial membrane potential was decreased in MG-63 cells after platycodin D treatment for 24 h. Conclusion Platycodin D can induce MG-63 cell apoptosis by enhancement of ROS level, which leads to mitochondria damage.