硝酸镧染毒对小鼠骨髓细胞微核率的影响

目的研究硝酸镧染毒对小鼠骨髓细胞微核率的影响。方法将21日龄昆明种小鼠随机分为9组,每组12只,分别腹部皮下注射不同剂量的硝酸镧溶液(10、50、100、200、300、400、500mg/kg),阴性对照组给予蒸馏水,阳性对照组给予环磷酰胺,每天1次,连续7d,观察小鼠骨髓细胞微核率及小鼠体重。结果300、400、500mg/kg组小鼠增重低于阴性对照组,差异有显著性(P<0.05)。100、200、300、400、500mg/kg组小鼠骨髓细胞微核率高于阴性对照组(P<0.05)。随染毒剂量增加,小鼠骨髓细胞微核率呈上升趋势(r=0.9223P<0.05)。结论硝酸镧具有一定遗传毒性。

Effects of Exposure to Lathanum Nitrate on Micronucleus Frequency of Bone Marrow Cells Among Mice

Objective To study the effects of exposure to lanthanum nitrate(LaNO3)on the micronucleus frequency of bone marrow cells among mice. Methods Kunming mice aged 21 days were randomly divided into 9 groups, 12 mice per group. The 9 groups included 7 LaNO3-treated groups at abdomenal area subcutaneously injected with LaNO3 solution at different doses of 10, 50, 100, 200, 300, 400, 500 mg/kg respectively, negative control group treated with distilled water and positive control group treated with cyclophosphamide. The treatment was performed once a day, continuously for 7 days. After 7-day treatment, the micronucleus frequency of bone marrow cells and body weight were measured among mice. Results The increases of body weights of mice in 300, 400 and 500 mg/kg LaNO3 exposure groups were significantly lower than those of negative control group(P<0.05). The micronucleus frequencies of bone marrow cells of mice in 100, 200, 300, 400 and 500 mg/kg groups were significantly higher than those of negative control groups(P<0.05). With the increase of the exposure doses of LaNO3, the micronucleus frequencies of bone marrow cells in mice increased gradually among mice (r=0.922 3, P<0.05). Conclusion LaNO3 presented a certain genotoxicity.