磷酸酶基因、核因子-κBp65、Survivin蛋白在子宫内膜样腺癌中的表达及其临床意义

目的探讨磷酸酶基因（PTEN）、核因子（NF-κB p65）及凋亡抑制蛋白Survivin基因在子宫内膜样腺癌中的表达及其在子宫内膜样腺癌的发生、发展中的作用。方法采用SP免疫组织化学法检测63例子宫内膜样腺癌、20例正常增生期子宫内膜组织中PTEN、NF-κB p65及Survivin蛋白的表达。结果子宫内膜样腺癌与正常增生期子宫内膜组织中PTEN、NF-κB p65及Survivin蛋白表达的阳性率差异有统计学意义（P〈0．01）。PTEN表达与子宫内膜样腺癌分化程度呈正相关（P〈0．001），与淋巴结转移及TNM分期呈负相关（P〈0．005），NF-κB p65的表达与子宫内膜样腺癌分化程度呈负相关（P〈0．05），与浸润深度、淋巴结转移及TNM分期呈正相关（P〈0．05）。Survivin与子宫内膜样腺癌与浸润深度、淋巴结转移及TNM分期呈正相关（P〈0．05）。结论PTEN、NF-κB p65及Survivin在子宫内膜腺癌的发生、发展中起着不同程度的作用，联合检测PTEN、NF-κB p65及Survivin对研究子宫内膜腺癌的发生、发展、早期诊断及预后评估有一定的参考价值。

Expression of PTEN,NF-κB p65 and Survivin in endometrial carcinoma and its clinical significance

Objective To explore the expression of PTEN, NF-κB p65 and Survivin protein and the influence in the genesis and development of endometrial carcinoma（EC）. Methods The expression of PTEN, NF-κB and Survivin in 63 cases of EC and 20 cases normal endometrial tissue specimens were detected by immunohistochemistry. Results There were obvious differences among the positive rates of PTEN, N-κB p65 and Survivin protein in EC compared with in normal endometrial tissue specimens （P 〈0.001）. The clinieopathological characteristics of endometrial carcinoma with PTEN, NF-κB p65 and Survivin were as follows： the expression of PTEN gene was positively correlated with the degree of histological differentiation（P 〈 0.001）, and it was negatively correlated with lymph node metastasis and TNM （P 〈0.005）. There was inverse correlation between the expression of NF-κB p65 gene and the degree of histological differentiation（P 〈0.05）. It was positively correlated with lymph node metastasis, deep myometrial invasion and TNM in EC （P 〈0.05）. The expression of Survivin was positively correlated with the clinical stage and lymph node metastasis and deep myometrial invasion in EC （P 〈0.05）. Conclusion There are different extent action of PTEN, NF-κB p65 and Survivin significantly in genesis and development of EC. Detection of PTEN combined with NF-κB p65 and Survivin is valuable for early diagnosing and evaluating malignancy extent of EC.