AmpliSensor-聚合酶链反应技术检测结核分支杆菌及临床应用

目的探讨Ａｍｐｌｉｓｅｎｓｏｒ－聚合酶链反应（ＡｍｐｌｉＳｅｎｓｏｒ－ＰＣＲ）在结核病诊断中的价值。方法采用ＡｍｐｌｉＳｅｎｓｏｒ－ＰＣＲ对７８４例结核病患者及１６０例肺癌患者的标本进行检测，并与ＰＣＲ（凝胶电泳后，经溴化乙锭染色）、涂片、培养等法比较。结果ＡｍｐｌｉＳｅｎｓｏｒ－ＰＣＲ的敏感性显著高于涂片及培养（Ｐ＜０．０１）。特异性较ＰＣＲ法高。结论ＡｍｐｌｉＳｅｎｓｏｒ－ＰＣＲ可以通过标准曲线划定检出下限，并可换算出标本中原始的靶ＤＮＡ值，同时具有较高的特异性和敏感性，对肺结核尤其是肺外结核的诊断有一定的临床意义。

Detection of Mycobacterium tuberculosis by AmpliSensor PCR technique and its clinical application

Objective To explore the value of Amplisensor PCR method in diagnosis of tuberculosis. Method The samples from 784 patients with tuberculosis, 160 patients with lung cancer were detected by using AmpliSensor PCR, PCR, smear and culture methods, and the results were compared. Result The sensitivity of AmpliSensor PCR technique was obviously higher than that of smear and culture ( P <0.01). The specificity of AmpliSensor PCR method was higher than that of PCR method. Conclusion Amplisensor PCR method can be determined to detect the lower limit by standard curve, through which the original amount of target DNA in samples can be calculated. AmpliSensor PCR method shows higher specificity and sensitivity. It is of clinical value in diagnosis of pulmonary tuberculosis and particularly of extrapulmonary tuberculosis.