牙龈卟啉单胞菌对脐静脉血管内皮细胞产生sICAM-1的影响

目的：观察牙龈卟啉单胞菌（Porphyromonas gingivalis,P.gingivalis)对人类脐静脉血管内皮细胞（human umbilical vein endothelial cells,HUVECs)产生可溶性细胞间粘附分子－1（soluble intercellular adhesion molecule-1,sICAM-1)的影响。方法：应用厌氧袋法培养P.gingivalis，并用其感染HUVECs，采和酶联免疫吸附实验（enzyme-linked immunosorbent assay,ELISA)测定培养上清中sICAM-1的含量，结果：HUVECs基础表达少量的sICAM-1;P.gingivalis剂量依赖性增强HUVECs产生sICAM-1蛋白的水平；紫外线、超声波和65℃的热处理都不能抑制P.gingivalis的作用；sICAM-1蛋白水平在P.gingivalis刺激后的4h未见改变，增高的作用从刺激后的8h开始，在12h,16h和20h继续增加。结论：活的和灭活的P.gingivalis都剂量依赖性和时间依赖性地增强HUVECs产生sICAM-1,P.gingivalis可能同样诱导牙龈血管内皮细胞表达sICAM-1，升高的sICAM-1可能参与调节牙周病炎症反应和免疫反应过程。

Effect of porphyromonas gingivalis on soluble intercellular adhesion molecule-1 production by human umbilical vein endothelial cells

AIM :To observe the effect of Porphyromonas gingivalis ( P. gingivalis ) on soluble intercellular adhesion molecule-1 (sICAM-1) production by human umbilical vein endothelial cells (HUVECs). METHODS: Anaeropack method was employed to culture P. gingivalis and then HUVECs were co-cultured with P. gingivalis following by assaying the amount of sICAM-1 with enzyme-linked immunosorbent assay(ELISA)kit. RESULTS: P. gingivalis dose-dependently increased the production of sICAM-1 in HUVECs. The treatment of UV, sonication and heating did not inhibit the effects of P. gingivalis . The amount of sICAM-1 did not increase at 4h after P. gingivalis infection; elevated sICAM-1 was found at 8h, and then continued to increase at 12h, 16h and 20h after co-cultivation. CONCLUSION: P. gingivalis increased the production of sICAM-1 in HUVECs with a dose and time dependent manner. P. gingivalis might induce the gingival endothelial cells to express sICAM-1 and elevated sICAM-1 might be involved in the regulation of inflammatory and immunological reaction in periodontal disease.