康莱特注射液对胃癌患者细胞凋亡、增殖及T细胞亚群的影响

目的：探讨康莱特对胃癌细胞凋亡对增殖及胃癌患者T细胞亚群的影响。方法：进展期胃癌患者30例，随机分为3组（每组各10例）：A组（对照组）：手术前后常规给予全胃肠外营养（total parenteral nutrition,TPN)治疗；B组（康莱特治疗组）：术前5d及术后9d，给予康莱特注射液200mL/d静脉滴入加常规TPN治疗；C组（化疗组）：术前给予5d化疗，甲酰四氢叶酸钙（calcium folinatefor,CF)200mg及5氟脲嘧啶（5－FU）750mg/d静脉滴注加常规TPN治疗。分别于治疗前及术后1d,5d及10d，采集外周静脉血，应用免疫荧光法检测CD3^ 、CD4^ 、CD8^ T细胞亚群。手术中取胃癌病理组织，用末端转移酶介导的dUTP切口末端标记法和免疫组织化学染色法检测胃癌细胞的凋亡（AI）与增殖（PI）及二者之比（AI／PI）。结果：B组与C组相比较，胃癌细胞的AI、PI及AI／PI，无显著差异（P＞0．05）；B组与A组相比较，上 3种指标则具有显著差异（P＜0．01）。治疗前3组CD3^ 、CD4^ 、CD8^ T细胞亚群的百分率无显著差异（P＞0．05）；术后1d,5d3组CD3^ 、CD4^ 、CD8^ T细胞亚群的百分率差异显著（P＜0．01）；术后10d,CD3^ 、CD4^ 、CD8^ T细胞亚群的百分率，B组与A组以及B组与C组相比较，差异性分别为显著（P＜0．05）及非常显著（P＜0．01）。结论：康莱特可明显促进胃癌细胞凋亡和抑制其增殖，有助于提高围手术期患者的免疫功能。

The effects of Kang-Lai-Te on apoptosis and proliferation of advanced gastric cancer cells and T-cell subset in gastric cancer patients

Aim To explore the effects of Kang-Lai-Te on apoptosis and proliferation of advanced gastric cancer cells, and on T-cell subset in gastric cancer patients. Methods 30 cases of advancedgastric cancers patients were randomly divided into three groups(A,B and C), 10 cases each group. Group A(control group): total parental nutrition(TPN) was administered by intravenous drip(VD) before and after operation. Group B (treatment group) were treated with Kang-Lai-Te injection of 200 mL plus TPN VD. Group C (chemotherapy group) were treated with calcium folinatefor(CF) of 200 mg IV, 5-FU of 700mg/d plus TNP VD 5 days prior to opreperation and 9 days following operation. The percentages of CD3 +?CD4 + and CD8 + T-cell subset in peripheral venous blood were detected by immuno flurescence assay before operation and 1,5 and 10 days after operation.The apoptosistic index(AI), proliferative index(PI) of gastric cancer cells and ratio of AI and PI were detected by terminal transferase mediated dUTP nike end-labeling and immuno histochemical staining. Results AI, PI and their ratio had no significant difference between the group B and C (P>0.05). Above 3 indexes had significant difference between the group B and A (P<0.01). Before treatment, the percentages of CD3 +?CD4 + and CD8 + T-cell subset ratio had no significant difference among 3 groups (P>0.05); but had significant difference (P<0.01) on one and 5 days following operation. 10 days after operation the percentages of CD3 +?CD4 + and CD8 + T-cell subset ratio had significant difference (P<0.05) compared between groups B and A. The difference between group B and C was very notable (P<0.01). Conclusion Kang-Lai-Te can markedly enhance apoptosis of gastric cancer cells and inhibites their proliferation. These effects might contribute to improve the patient's immune state obviously.