不同等级白芍药材中芍药苷含量的分析

目的：测定不同等级白芍中芍药苷的含量。方法：采用反相HPLC法，色谱柱：KromasilC18（150mm×4．6mm，5μm）；流动相：乙腈旬．1％磷酸溶液（14：86）；柱温：30℃；流速：1．0mL·min-1；检测波长：230nm。结果：芍药苷进样量在0．2016～1．008μg线性关系良好（r=0．9999），平均回收率为97．36％（RSD=1．27％）。19批样品测定结果表明，一等品中芍药苷的含量在2．88％-3．54％，二等品中芍药苷的含量在2．46％～2．78％，三等品中芍药苷的含量在2．04％～2．32％，四等品中芍药苷的含量在1．34％～1．97％。结论：白芍现有的划分等级的方法较好地体现了白芍的质量差异，为白芍的临床使用和制药企业选择原料提供了依据。

Determination of Paeoniflorin in Paeoniae Radix Alba of Different Grades by HPLC

Objective： To established a method for the determination of paeoniflorin in Paeoniae Radix Alba.Methods： RP-HPLC method was on Kromasil C18 column （150 mm × 4. 6 mm, 5μm）, with acetonitrile-0. 1% phosphoric acid （14：86） as mobile phase and detection wavelength at 230 nm. The flow rate was 1.0 mL.min 1 and column temperature was 25℃. Results： The calibration curves of paeoniflorin were in good linearity at the range of 0. 201 6-1. 008 μg （r=0. 999 9） . The average recovery was 97.36% （n =6, RSD = 1.27% ） . The results from 19 samples showed that the content of paeoniflorin was between 2. 88% to 3.54% in first rank sample, 2.46% to 2. 78% in second rank sample, 2. 04% to 2.32% in third rank sample, 1.34% to 1.97% in fourth rank sample, respectively. Conclusion： The classification properly reflects the quality of Paeoniae Radix Alba according to content of paeoniflorin, and provides quality information for clinical use.