大鼠重症急性胰腺炎肺泡巨噬细胞的活化和调控作用

目的 观察巨噬细胞中p38蛋白活化激酶对大鼠重症急性胰腺炎肺损伤的影响.方法 假手术组仅胆胰管注射生理盐水0.1 ml/100 g;5%的牛黄胆酸(0.1 ml/100 g)逆行注射到SD大鼠的胆胰管内,造成重症急性胰腺炎(SAP)分为SAP组和SB03580组(SB203580,0.5 mg/kg,静注).在6 h剖杀大鼠,查腹水,抽血检测血清淀粉酶(AMS)和肿瘤坏死因子(TNF)-α和白细胞介素(IL)-6[酶联免疫吸附试验(ELISA)法];同时分别离心收集全肺肺泡巨噬细胞,免疫组织化学检测肺组织及其巨噬细胞中p38 MAPK的表达,同时检测肺组织中的TNF-α和IL-6;光镜下检测胰腺组织损害.结果 SAP组及SB组中AMS在6 h分别为(4865.12±890.35)IU/L和(2918.24±614.58)IU/L,差异有统计学意义.血清TNF-α分别为(106.59±43.71)ng/L和(76.43±38.43)ng/L;IL-6是(2203.76±640.85)ng/L和(1254.76±459.35)ng/L,差异有统计学意义(P<0.01).肺组织中的TNF-α和IL-6的升高与血中的一致.光镜下,胰腺组织内可见炎细胞浸润、充血、水肿和坏死;在SAP组中肺组织及其巨噬细胞中p38 MAPK强烈表达,TNF-α和IL-6的表达一致,SB治疗组表达下降.结论 TNF-α和IL-6在重症急性胰腺炎肺损伤起着重要作用;肺泡巨噬细胞中p38 MAPK表达对TNF-α和IL-6的转录和合成起重要作用.

The activation of rat alveolar macrophages in rats with severe acute pancreatitis

Objective To investigate the role of p38 protein kinase in the activation of rat alveo-lar macrophages (AMs) in rats with severe acute pancreatitis (SAP). Methods Sixty Spragne Dawley rats were anesthetized, SAP was induced by low pressure retrograde infusion of biliopancreastic duct with 5% sodium tanrocholate solution (0.1 ml/100 g), and the rats were divided into SAP group and SB203580 group (SB203580,0.5 mg/kg, i. v). Thirty Sprague Dawley rats were injected with normal solu-tion into biliopancreastic duct (0.1 ml/100 g). Six h after operation,the rats were sacrificed. The serum and lung levels of TNF-α/IL-6 were determined by ELISA. The expression pattern of p38 MAPK in lung tissues and AMs was detected by immunohistochemistry. Results The levels of TNF-α/IL-6 and AMs in SAP group and SB group were increased more significantly than in sham-operated group (P<0.001). The expression of p38MAPK in lung tissues and AMs was more significantly increased in SAP group and blocked by SB203580. Conclusion The serum levels of TNF-α/IL-6 in SAP rats were assoeiated with se-verity of SAP. The induction of TNF-α and IL-6 in lung tissues and AMs may be mediated through the acti-vation of p38 MAPK.