血管化可注射性纳米组织工程骨对骨再生血管形成的影响

目的将血管化可注射性纳米组织工程骨注入骨延长区，观察血管形成及骨再生的情况，揭示组织工程骨再血管化和成骨的关系。方法以可注射性纳米羟基磷灰石胶原（NHAC）／藻酸盐水凝胶为载体，复合成骨细胞和血管内皮细胞构建血管化可注射性纳米组织工程骨，将其注入肢体延长动物模型骨延长区，以空白对照组、注射单纯成骨细胞复合可注射性纳米材料组为对照，通过组织学切片、毛细血管计数、骨小梁百分比面积测定，观察血管化及骨生成情况。结果毛细血管计数显示第3周：A组4．8±9．1，B组7．4±9．2，C组10．7±8．5；第6周：A组15．1±7．2，B组19．3±15．8，C组31．5±18．2，各时段3组间均有统计学意义（P〈0．05），c组血管计数值最高，血管化明显优于其他组。骨小梁百分比面积测定显示第3周：A组4．52±7．31，B组10．20±9．65，C组21．33±16．4；第6周：A组22．56±8．76，B组41．95±16．27，C组58．36±11．39，各时段3组间均有统计学意义（P〈0．05），数值由高到低顺序排列为：C组〉B组〉A组，C组骨小梁百分比面积最大。结论血管化可注射性纳米组织工程骨应用于牵拉成骨，可促进骨延长区血管化及骨生成，优于单纯成骨细胞复合可注射性纳米材料。

Effect of the injectable nano-vascularized tissue-engineered bone on bone regeneration angiogenesis

Objective To observe bone angiogenesis and regeneration and to reveal the relationship of tissue engineered bone revascularization and osteogenesis after the application of the injectable nano-vascularized tissue- engineered bone. Methods To construct vascularization injectable nano tissue engineered bone with osteoblasts and endothelial cells and apply in distraction osteogenesis. Observed the angiogenesis and osteogenesis by histological examination,capillaries count, bone trabecula area percent after the application of the injectable nano-vascularized tissue-engineered bone in group C. At the same time compared with the empty control group- group A and the simple osteoblasts composite injectable nano tissue-engineered bone group-group B. Results Capillary counting display in third weeks ： group A 4. 8 ±9. 1, group B 7.4 ± 9. 2, group C 10. 7 ± 8. 5 ; Capillary counting display in sixth weeks ： group A 15.1 ±7.2,group B 19. 3 ± 15.8, group C 31.5 ± 18. 2. Two periods of time between the 3 groups were statistically significant（P 〈 0.05 ）. Group C vessel count value maximum. Vascularization was superior to the other group. In third weeks, the percentage area of trabecular bone ： group A 4. 52 ± 7.31, group B 10. 20 ± 9. 65, group C 21.33± 16.4; In sixth weeks, the percentage area of trabecular bone： group A 22.56 ± 8.76, group B 41.95 ± 16.27 ,group C 58.36 ± 11.39. Two periods of time between the 3 groups were statistically significant（ P 〈 0. 05）. Value from high to low order was group C to group B then to group A . Group C trabecular bone area was the largest percentage. Conclusion The injectable nano-vascularized tissue-engineered bone can accelerate the angiogenesis and osteogenesis and excelled the simple osteoblasts composite injectable nano tissue-engineered bone.