川芎嗪对大鼠肝脏缺血-再灌注损伤保护作用的机理研究

目的　研究川芎嗪注射液对大鼠肝脏缺血再灌注损伤的保护作用。方法　96 只SD大鼠被随机均分为假手术组、肝脏缺血再灌注组(I/R组)和肝脏缺血+川芎嗪再灌注组(简称治疗组)3 组,分别于术前及术后30 min、6 h及24 h检测血浆丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)及乳酸脱氢酶(LDH),观察每组大鼠1周存活情况及肝脏病理组织学变化,并行肝细胞凋亡指数检测。结果　治疗组术后1 周大鼠存活情况好于I/R组(P<0.05); 治疗组及I/R组血浆ALT、AST及LDH均明显高于假手术组(P<0.05),但治疗组低于I/R组(P<0.05)。光镜下见,缺血再灌注后肝血窦和中央静脉明显瘀血,肝细胞坏死I/R组重于治疗组。肝细胞凋亡指数I/R组高于治疗组(P<0.05)。结论　川芎嗪对大鼠肝脏缺血再灌注损伤具有明显的保护作用。

Protective Effect of Ligustrazine on Ischemia-Reperfusion Injury of Rat Liver

Objective To explore the protective effect of ligustrazine on the ischemia-reperfusion injury of rat liver. Methods Ninety-six healthy SD rats were divided randomly into three groups: sham operation group, ischemia-reperfusion group(I/R group) and ischemia plus ligustrazine reperfusion group(therapy group).The plasm ALT,AST and LDH were measured before operation,at thirty minutes,six hours and twenty-four hours after operation. One week survival and liver pathological change of every group were observed, and the hepatocyte apoptosis index was measured simultaneously.Results One week survival of therapy group was higher than that of I/R group ( P <0.05). The plasm ALT,AST and LDH of therapy group and I/R group were higher than those of the sham operation group significantly ( P <0.05), and those of therapy group were lower than those of the I/R group ( P < 0.05). Light microscopy indicated that the liver sinusoid and central veins were congested remarkably after operation, the hepatocyte necrosis in I/R group was more severe than that in therapy group, and the hepatocyte apoptosis index of I/R group was higher than that of therapy group ( P <0.05). Conclusion The protective effect of ligustrazine on ischemia-reperfusion injury of rat liver is obvious.