铜绿假单胞菌定量冷冻干燥菌球与新鲜制备菌悬液用于胰酪大豆胨琼脂培养基适用性检查的研究

目的：探讨使用铜绿假单胞菌CMCC(B)10104定量冷冻干燥菌球制备菌悬液(以下简称冻干型菌悬液)与新鲜制备菌悬液(以下简称新鲜型菌悬液)评价胰酪大豆胨琼脂培养基适用性的差异。方法：将铜绿假单胞菌CMCC(B)10104冻干型菌悬液和新鲜型菌悬液分别涂布5种市售胰酪大豆胨琼脂培养基，获得培养物的菌落数量、菌落平均直径及肉眼可见菌落出现时间，使用SPSS软件进行统计学分析。结果：铜绿假单胞菌CMCC(B)10104冻干型菌悬液与新鲜型菌悬液的差异主要体现在菌落平均直径上，前者大于后者。铜绿假单胞菌CMCC(B)10104冻干型菌悬液在不同品牌胰酪大豆胨琼脂培养基平板上菌落计数明显不同。结论：使用铜绿假单胞菌CMCC(B)10104冻干型菌悬液更利于筛选对损伤菌促生长能力强的培养基，可以用于计数培养基(胰酪大豆胨琼脂培养基)适用性检查。

Study on the Applicability Test of Quantitative Lyophilized Ball and Freshly Prepared Suspension of Pseudomonas aeruginosa for the Evaluation of Tryptic Soy Agar

Objective: To investigate the difference between quantitative lyophilized ball suspension and freshly prepared suspension of Pseudomonas aeruginosa CMCC (B) 10104 for evaluating the applicability of Tryptic Soy Agar. Methods: Five types of commercially vailable Tryptic Soy Agar were inoculated with quantitative lyophilized ball suspension and freshly prepared suspension of Pseudomonas aeruginosa CMCC (B) 10104 respectively. The number of colonies, average diameter of colonies and appearance time of colonies were obtained. The differences were analyzed by SPSS statistical software. Results: The difference between lyophilized ball suspension and freshly prepared suspension of Pseudomonas aeruginosa CMCC (B) 10104 was mainly refl ected in the average diameter of colonies. The average diameter of colonies of the lyophilized ball suspension was larger than that of freshly prepared suspension. The colony count of Pseudomonas aeruginosa CMCC (B) 10104 lyophilized ball suspension was significantly different on different brands of Tryptic Soy Agar plates. Conclusion: Using the lyophilized ball suspension of Pseudomonas aeruginosa CMCC (B) 10104 is more conducive to screening the medium with strong growth promoting ability to the damaged bacteria, and could be used to check the applicability of counting medium (Tryptic Soy Agar).