| 安徽药菊茎尖组织培养技术的研究 |
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| 引用本文： | 薛建平,张爱民,盛玮,赵丰兰.安徽药菊茎尖组织培养技术的研究[J].中国中药杂志,2002,27(5):350-352. |
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| 作者姓名： | 薛建平 张爱民 盛玮 赵丰兰 |
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| 作者单位： | 淮北煤炭师范学院,生物系,安徽,淮北,235000 |
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| 基金项目： | 安徽省“十五”重大科技专项 (0 180 3 0 0 4) |
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| 摘 要： | 目的 :研究安徽药菊茎尖组织培养技术 ,建立最佳培养条件。方法 :取药菊的茎尖 0.5mm左右 ,分别在不同的培养基中培养 ,诱导其为完整植株。结果与结论 :以MS+6-BA 2mg·L-1+NAA 0.2mg·L-1为茎尖诱导培养基较好 ,40d后 ,芽诱导率达 80%以上 ;以MS+6BA2mg·L-1+NAA0.5mg·L-1为芽增殖培养基 ,25～30d后 ,芽增殖系数达 4～7倍 ;生根培养基以MS +NAA 0.5mg·L-1为宜 ,生根率 100% ,根系粗壮 ,移栽易于成活。
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| 关 键 词： | 药用菊花 茎尖 组织培养 |
| 文章编号： | 1001-5302(2002)05-0350-03 |
| 收稿时间： | 2001/10/30 0:00:00 |
| 修稿时间： | 2001年10月30 |
Study on Stem-tip Tissue Culture of the Traditional Chinese Medicine Chrysanthemum morifolium |
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| XUE Jian ping;ZHANG Ai min;SHENG wei;ZHAO Feng lan.Study on Stem-tip Tissue Culture of the Traditional Chinese Medicine Chrysanthemum morifolium[J].China Journal of Chinese Materia Medica,2002,27(5):350-352. |
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| Authors: | XUE Jian ping;ZHANG Ai min;SHENG wei;ZHAO Feng lan |
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| Institution: | Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235000, Anhui, China. |
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| Abstract: | OBJECTIVE: To set up the optimums for the stem-tip tissue culture of Chrysanthemum morifolium cultivated in Anhui Province. METHOD: Small sections (about 0.5 mm in length) from the stem-tips were isolated and inoculated with different media, and induced to form the whole plantlet formation. RESULT AND CONCLUSION: The MS medium added with 6-BA 2 mg.L-1 + NAA 0.2 mg.L-1 was the optimum medium for the bud sprouting and the inducing rate was over 80% after 40 d cultivation on this modified medium. The MS medium supplemented with 6-BA 2 mg.L-1 and NAA 0.5 mg.L-1 was the optimum medium for the multiplication of the adventitious buds in which the bud multiplication was about 4-7 times higher after 25-30 d cultivation. The plantlet could root well on the MS medium with NAA 0.5 mg.L-1. |
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| Keywords: | Chrysanthemum morifolium stem tip tissue culture |
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