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| 细胞凋亡检测用于卵巢癌化疗药物敏感性试验及其与ATP法的比较 | |
| 引用本文： | 楼江燕 王和 等. 细胞凋亡检测用于卵巢癌化疗药物敏感性试验及其与ATP法的比较[J]. 华西医科大学学报, 2002, 33(2): 267-269 |
| 作者姓名： | 楼江燕 王和 等 |
| 摘 要： | 目的 探讨细胞凋亡检测用于卵巢癌化疗药物敏感性试验的可行性。方法 采用末端标记法直接检测顺氯胺铂等6种化疗药物诱导4例卵巢癌实体瘤细胞凋亡的结果。并与三磷酸腺苷生物发光法（ATP法）检测结果进行对比。结果 同一种化疗药物对不同来源的癌细胞的细胞凋亡诱导程度不同，与ATP法比较二者的检测结果存在一致性（P0=0.75)。结论 上述结果提示，细胞凋亡检测能直接用于 了解药物诱导肿瘤细胞凋亡的程度，若作为一种药物敏感性试验方法与ATP法组合应用，将有可能提高临床应用效果。 |
| 关 键 词： | 细胞凋亡 卵巢癌 化疗药物敏感性试验 ATP法 比较 三磷酸腺苷生物发光法 |
Comparison between apoptosis cells count and ATP-bioluminescence assay in ovarian cancer chemosensitivity testing in vitro] |
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| Jiangyan Lou,He Wang,Shanling Liu,Zhilan Peng. Comparison between apoptosis cells count and ATP-bioluminescence assay in ovarian cancer chemosensitivity testing in vitro][J]. Journal of West China University of Medical Sciences, 2002, 33(2): 267-269 | |
| Authors: | Jiangyan Lou He Wang Shanling Liu Zhilan Peng |
| Affiliation: | Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China. |
| Abstract: | OBJECTIVE: This study was aimed to evaluate the potentiality for the use of apoptosis cells count as a chemosensitivity testing in vitro for ovarian cancer. METHODS: An end-labelling assay was used to determine the apoptosis cells of 4 specimens of fresh ovarian cancer cells and the results were compared with that of ATP assay. RESULTS: It was found that chemotherapy can induce apoptosis, and the same chemotherapeutic agent can induce different apoptosis cells for different cancers. CONCLUSION: The observed agreement between apoptosis cells count and ATP assay was 0.75. The above data demonstrate that apoptosis cells count can be used as a chemosensitivity testing for ovarian cancer, and a combination of these two methods may improve the clinical effects of chemotherapeutic agents. |
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