| 抗胃转移性腺癌细胞系单克隆抗体的制备及鉴定 |
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| 引用本文： | 李成刚,林三仁,杨雪玲,丁晔,王立新. 抗胃转移性腺癌细胞系单克隆抗体的制备及鉴定[J]. 北京大学学报(医学版), 1994, 0(6) |
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| 作者姓名： | 李成刚 林三仁 杨雪玲 丁晔 王立新 |
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| 作者单位： | 北京医科大学第二医院消化疾病研究中心，辽宁省抚顺市第二医院内科 |
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| 摘 要： | 用胃转移性腺癌细胞系ＳＧＣ７９０１免疫ＢＡＬＢ／Ｃ小鼠，取脾与ＳＰ２／０骨髓瘤细胞融合，筛选出４株抗胃癌单克隆抗体，分别命名为ＢＭＧ１，ＢＭＧ２、ＢＭＧ３和ＢＭＧ４。经间接细胞免疫荧光和ＡＢＣ免疫组化染色鉴定，这组单抗与胃癌细胞系和胃癌组织有高的反应性，其中ＢＭＧ１、ＢＭＧ３与中分化腺癌及低分比腺癌的反应性分别为８４．６％和８３．３％；与正常胃粘膜腺体不反应；与部分异型增生腺体呈阳性反应。初步认为，此组单抗在胃癌的组织学和体液诊断方面有一定应用价值。
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| 关 键 词： | 胃癌，单克隆抗体，制备 |
PRODUCTION AND CHARACTERIZATIPN OF FOUR MONOCLONAL ANTIBODIES TO HUMAn GASTRIC ADENOCARCINOMA CELL LINE |
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| Digestive Disease Research Center,The Third Hospital Li Chenggang,et al. PRODUCTION AND CHARACTERIZATIPN OF FOUR MONOCLONAL ANTIBODIES TO HUMAn GASTRIC ADENOCARCINOMA CELL LINE[J]. Journal of Peking University. Health sciences, 1994, 0(6) |
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| Authors: | Digestive Disease Research Center The Third Hospital Li Chenggang et al |
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| Abstract: | our monoclonal antibodies disignated BMGs were generated by immunizing BALB/c mice with the SGC7901,A gastric adenocarcinoma cell line. 123 cases of gastric specimen were studied by immu- nohistochemistry. BMG1 and BMG4 reacted with 77.8%and 81.3%of the well differentiated adenoca- cinoma,moderately differentiated adenocarcinoma and the reactivity of 84.6%and 66.7%,respecti- vely;83.3%of poorly differentiated adenocarcinoma and 71.4%of mucinous adenocarcinoma were stained by BMG3.The MAbs did not react with normal gastric tissues.The corresponding antigens were consi- dered as glycoproteins expressed on the cell membrane. |
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| Keywords: | Gastric carcinoma Monoclonal antibody Preparation |
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