益气解毒方基于线粒体保护的抗缺血性脑卒中研究

目的:基于线粒体呼吸功能,研究益气解毒方对缺血性脑卒中的保护作用。方法:采取大鼠中脑动脉闭塞模型(Middle Cerebral Artery Occlusion,MCAO),造模前15 min给予益气解毒方及相应拆方,造模12 h后,取材,提取脑组织线粒体,检测线粒体三态呼吸速率、四态呼吸速率、磷氧比、呼吸控制率、氧化磷酸化率等指标,评价益气解毒方对缺血性脑卒中的保护效果。此外,通过主成份分析(Principal Component Analysis,PCA)和变量重要性指标(Variable Importance in Projection,VIP),评价益气解毒方各组分在抗缺血性脑卒中的作用效果。结果:益气解毒方能改善缺血性脑卒中病理过程中线粒体呼吸相关的各项指标,益气解毒方各拆方对缺血性脑卒中也有较好的改善作用。PCA结果显示,与各拆方组比较,益气解毒方全方的治疗效果最好;在各拆方组中,人参皂苷和黄连素配伍后的效果最好。VIP分析结果显示,益气解毒方基于保护线粒体呼吸功能来改善缺血性脑卒中的病理过程中,黄连素的贡献度最大。结论:益气解毒方能够显著降低缺血性脑卒中病理过程中所造成机体损伤,其机制可能与保护线粒体功能有关。

Study on Effects of Yiqi Jiedu Fang on Mitochondrial Metabolism-related Energy Function in Ischemic Stroke Rats

To study the effects of Yiqi Jiedu Fang on mitochondrial energy metabolism related functions in ischemic stroke rats, and to explore the possible protective mechanisms of Yiqi Jiedu Fang on ischemic stroke through intervening the mitochondrial energy metabolism disorder.Methods:Middle Cerebral Artery Occlusion (MCAO) was used. Yiqi Jiedu Fang and its separated formulas were given 15 min prior to MCAO. After 12 h of modeling, mitochondria were extracted. Indexes of mitochondrial three-state respiration rate, four-state respiratory rate, phosphorus-oxygen ratio, respiratory control rate, oxidative phosphorylation rate and other indicators were determined. The protective effect of Yiqi Jiedu Fang on ischemic stroke was evaluated. In addition, the effect of each component of Yiqi Jiedu Fang on anti-ischemic stroke was evaluated by Principal Component Analysis (PCA) and Variable Importance in Projection (VIP).Results:Yiqi Jiedu Fang can improve the mitochondrial respiration related indexes during the pathological process of ischemic stroke. The separated formula of Yiqi Jiedu Fang also has a better effect on ischemic stroke. PCA results showed that compared with other separated formula groups, Yiqi Jiedu Fang group had the best treatment effect. In each separated formula groups, the compatibility of Radix et Rhizoma Ginseng, Fructus Gleditsiae and berberine had the best treatment effect. The results of VIP analysis showed that berberine had the largest contribution to the pathological process of ischemic stroke based on the protection of mitochondrial respiratory function.Conclusion:Yiqi Jiedu Fang can significantly reduce the pathological damage caused by ischemic stroke, whose mechanism may be related to the protection of mitochondrial function.