肝硬化脾行脾次全切除术前后脾脏基质金属蛋白酶及其抑制剂的表达

目的 了解肝硬化充血性脾肿大伴脾亢时行脾次全切除术前后脾脏基质金属蛋白酶及其抑制剂的表达变化。方法 用60％CCl4加5％乙醇制作大鼠肝纤维化模型，然后行脾次全切除术，用原位杂交和免疫组化的方法观察手术前后脾脏中膜型基质金属蛋白酶－1（MT1－MMP）、基质金属蛋白酶2（MMP2）、基质金属蛋白酶抑制剂－1（TIMP1）的表达。结果 原位杂交显示，脾脏中MT1－MMP、MMP2、TIMP1 mRNA的表达主要在血管内皮和血窦内皮细胞、成纤维细胞及网状细胞中，多位于红髓、脾索和脾小梁。蓝紫色阳性物质分布于胞质中。图像分析表明手术前后比较无明显差异（P＞0.05)。MMP9的免疫组化染色显示，棕黄色的阳性物质主要分布在红髓和边缘带的吞噬细胞和中性粒细胞中，图像分析表明手术前后比较均无统计学差异（P＞0.05)。结论 脾次全切除术后保留的残脾其纤维化程度未见加重。

Expression of TIMP and MMP in spleen of cirrhotic rats before and after subtotal splenectomy

Objective To explore the expressions of tissue inhibitor of metalloproteinase (TIMP) and matrix metalloproteinase (MMP) in the spleen of cirrhotic rats with splenomegaly and hypersplenism before and after subtotal splenectomy. Methods Cirrhosis of liver was induced with 60% carbon tetrachloride and 5% alcohol in rats, then subtotal splenectomy was performed. The levels and cellular locations of TIMP1, MT1 MMP, MMP9, and MMP2 in the spleen of splenomegaly and hypersplenism before and after subtotal splenectomy were determind with immunohistochemistry and in situ hybridization. Results The amounts of TIMP1, MT1 MMP, MMP9, and MMP2 expressions had no significant differences in the spleen of cirrhotic rats with splenomegaly and hypersplenism before and after subtotal splenectomy ( P >0.05). Conclusion Subtotal splenectomy did not enhance the fibrosis of the residual splenic tissue.