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| 携带人c-Myc和EGFP基因慢病毒表达载体的构建 | |
| 引用本文： | 李艳青,史俊文,肖高芳,肖东,姚开泰. 携带人c-Myc和EGFP基因慢病毒表达载体的构建[J]. 热带医学杂志, 2011, 11(4): 369-371 |
| 作者姓名： | 李艳青 史俊文 肖高芳 肖东 姚开泰 |
| 作者单位： | 1. 南方医科大学肿瘤研究所,广东,广州,510515 2. 南方医科大学肿瘤研究所,广东,广州,510515;南方医科大学比较医学研究所暨实验动物中心,广东,广州,510515 |
| 基金项目： | 国家自然科学基金委员会-广东省联合基金重点项目，国家自然科学基金，广东省科技计划项目，广东省自然科学基金，广东省卫生厅基金，南方医科大学优秀中青年科技人才库科研资助金 |
| 摘 要： | 目的 构建携带人c-Myc和EGFP基因的慢病毒表达载体pFUMGW.方法 Xho I线性化plenti-EFla-c-Myc-IRES-EGFP,回收片段并补平Xho I,接着BamH I酶切该片段,回收2722 bp片段而获连接用c-Myc-IRES-EGFP;EcoR I线性化pFUGW,回收并补平EcoR I... |
| 关 键 词： | c-Myc EGFP 慢病毒载体 |
Construction of a lentiviral expression vector harboring human c-Myc and EGFP genes |
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| LI Yan-qing,SHI Jun-wen,XIAO Gao-fang,XIAO Dong,YAO Kai-tai. Construction of a lentiviral expression vector harboring human c-Myc and EGFP genes[J]. Journal Of Tropical Medicine, 2011, 11(4): 369-371 | |
| Authors: | LI Yan-qing SHI Jun-wen XIAO Gao-fang XIAO Dong YAO Kai-tai |
| Affiliation: | LI Yan-qing1,SHI Jun-wen1,XIAO Gao-fang1,XIAO Dong1,2,YAO Kai-tai1 (1.Cancer Research Institute,Southern Medical University,Guangdong,Guangzhou 510515,2.Institute of Comparative Medicine and Center of Experimental Animals,China) |
| Abstract: | Objective To generate lentiviral expression vector harboring human c-Myc gene and EGFP gene.Methods c-Myc-IRES-EGFP was released from pLenti-EF1a-c-Myc-IRES-EGFP by digestion with Xho I,blunted at Xho I site,and then by digestion with BamH I,and subsequently directionally cloned into the restriction sites EcoR I(blunted at EcoR I site)and BamH I of the lentiviral vector pFUGW after EGFP gene was released from pFUGW by digestion with EcoR I,blunted at EcoR I site,and then by digestion with BamH I,designated ... |
| Keywords: | c-Myc EGFP |
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