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| 光照应激对SD大鼠睾丸中Annexin 5表达的影响 | |
| 引用本文： | 时姗姗,王大勇,柳海燕,陶晓倩,韩雪峰,卢坤刚,张艳梅,戈一峰,崔英霞,姚兵. 光照应激对SD大鼠睾丸中Annexin 5表达的影响[J]. 解放军医学杂志, 2010, 35(2) |
| 作者姓名： | 时姗姗 王大勇 柳海燕 陶晓倩 韩雪峰 卢坤刚 张艳梅 戈一峰 崔英霞 姚兵 |
| 作者单位： | 1. 南京军区南京总医院解放军临床检验医学院研究所,南京,210002 2. 南京军区联勤部卫生部,南京,210016 |
| 基金项目： | 国家自然科学基金项目(30770801);;全军“十一五”医药卫生科研基金资助项目(06H011) |
| 摘 要： | 目的观察光照应激状态下SD大鼠睾丸组织中annexin5的表达变化。方法建立SD大鼠光照应激模型,24h持续光照,分为光照应激短期(2、3、4、7d)和光照应激长期(14、21、28d),分别取应激状态和相应对照组的大鼠睾丸组织,采用免疫组织化学和Western blotting技术分析annexin5免疫定位和蛋白相对表达。结果Western blotting结果显示:短期光照应激组(2、3、4、7d)的实验组大鼠睾丸annexin5表达与对照组相比无明显变化;在持续光照14d和21d后,实验组大鼠睾丸annexin5的相对表达量与对照组相比分别从3.09提高到4.16,从4.20提高到5.28(P<0.01,P<0.05);而持续光照28d后,实验组大鼠睾丸annexin5的相对表达量(4.87)与对照组(4.13)比较无明显差异(P>0.05)。免疫组织化学显示,annexin5在大鼠睾丸中的分布未见明显改变,但光照应激长期实验组(14、21、28d)annexin5免疫组化显色较深,说明annexin5表达升高,这与Westernblotting的结果基本一致。结论长期光照应激使大鼠睾丸annexin... |
| 关 键 词： | 光 应激 Annexin 5 基因 |
Effect of constant light stress on expression of Annexin 5 in testis of SD rats |
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| SHI Shan-shan,WANG Da-yong,LIU Hai-yan,TAO Xiao-qian,HAN Xue-feng,LU Kun-gang,ZHANG Yan-mei,GE Yi-feng,CUI Ying-xia,YAO Bing. Effect of constant light stress on expression of Annexin 5 in testis of SD rats[J]. Medical Journal of Chinese People's Liberation Army, 2010, 35(2) | |
| Authors: | SHI Shan-shan WANG Da-yong LIU Hai-yan TAO Xiao-qian HAN Xue-feng LU Kun-gang ZHANG Yan-mei GE Yi-feng CUI Ying-xia YAO Bing |
| Abstract: | Objective To observe the expression of annexin 5 in testis of SD rats under constant light stress.MethodsSD rats were exposed to constant light to establish short(2d,3d,4d,7d)and long-term (14d,21d,28d) constant-light-stressed models.The testes were then obtained from the model rats and their corresponding controls respectively.Immunolocalization and relative expression of annexin 5 in rats' testes were analyzed by immunohistochemistry and Western blotting.ResultsWestern blotting showed that,compared with t... |
| Keywords: | light stress Annexin 5 gene |
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