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| 重组人骨形成蛋白-2在诱导C3H10T1/2干细胞成骨中对MMP-9 mRNA表达的影响 | |
| 引用本文： | 徐岚,姜智,仇灏,史宁,吴士良. 重组人骨形成蛋白-2在诱导C3H10T1/2干细胞成骨中对MMP-9 mRNA表达的影响[J]. 江苏大学学报(医学版), 2004, 14(5): 402-404 |
| 作者姓名： | 徐岚 姜智 仇灏 史宁 吴士良 |
| 作者单位： | 苏州大学医学院生物化学与分子生物学教研室,苏州大学生化工程研究所,江苏,苏州,215007;苏州大学医学院生物化学与分子生物学教研室,苏州大学生化工程研究所,江苏,苏州,215007;苏州大学医学院生物化学与分子生物学教研室,苏州大学生化工程研究所,江苏,苏州,215007;苏州大学医学院生物化学与分子生物学教研室,苏州大学生化工程研究所,江苏,苏州,215007;苏州大学医学院生物化学与分子生物学教研室,苏州大学生化工程研究所,江苏,苏州,215007 |
| 摘 要： | 目的：测定MMP-9mRNA在C3H10T1／2 clone 8(10T1／2)中的表达，探讨rhBMP-2诱导10T1／2产生成骨细胞时对MMP-9mRNA表达的影响。方法：取10T1／2培养至细胞完全贴壁，加入rhBMP-2培养28天，同时利用Real—time PCR检测MMP-9在10T1／2中不同时间段的表达。结果：10T1／2经rhBMP-2的诱导14天出现钙化点，MMP-9mRNA也随着rhBMP-2的加入表达降低，而对照组几乎不变化。结论：rhBMP-2在诱导10T1／2产生成骨细胞的同时抑制着MMP-9mRNA的表达。 |
| 关 键 词： | MMP-9 重组人骨形成蛋白-2 C3H10T1/2 Real-time PCR |
| 文章编号： | 1671-7783(2004)05-0402-03 |
| 修稿时间： | 2004-09-16 |
Effect of rhBMP-2 on the Expression of mRNAs for Matrix Metalloproteinase-9 During rhBMP-2 Induced Osteogenic Differentiation of C3H10T1/2 Sterm Cells |
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| XU Lan,JIANG Zhi,QIU Hao,SHI Ning,WU Shi-liang. Effect of rhBMP-2 on the Expression of mRNAs for Matrix Metalloproteinase-9 During rhBMP-2 Induced Osteogenic Differentiation of C3H10T1/2 Sterm Cells[J]. Journal of Jiangsu University Medicine Edition, 2004, 14(5): 402-404 | |
| Authors: | XU Lan JIANG Zhi QIU Hao SHI Ning WU Shi-liang |
| Abstract: | Objective: To detect the expression of MMP-9 gene s in C3H10T1/2 clone 8(10T1/2) cells and study the effect of rhBMP-2 on MMP-9 during rhBMP-2 induced osteogenic differentation. Methods: rhBMP -2 was added to monolayer 10T1/2 cells, then the treated 10T1/2 cells were cu ltured for 28 days, Meanwhile, Real-time PCR was used to detect the expression of MMP-9 genes in the 10T1/2 cells at different times. Results: Calcified spots appeared when the 10T1/2 cells were induced by rhBMP-2 for 14 days.The expression of MMP-9 decreased due to the addition of rhBMP-2 while th e control group changed little. Conclusion: rhBMP-2 not only inhi bit the activity of MMP-9 but also induces osteogenic differentiation of 10T1/ 2 cells. [ |
| Keywords: | MMP-9 rhBMP-2 C3H10T1/2 Real-time PCR |
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