ConA诱发昆明小鼠T细胞依赖性肝脏损害

目的制作ConA引发昆明小鼠T细胞介导的肝损害方法参考Tiegsetal工作的基础上，本文经尾静脉将ConA2mL（10，20，40mg／Kg）注入昆明小鼠（n＝6）体内．8h后测定血清ALT及肝组织MDA，并作肝组织HE染色，病理学检查．对照组（n＝6）仅采用溶剂PBS2mL另外，用环胞素A（CSA）130mg／kg预处理（－15h，－1h），并作同样检查，观察预防效果．结果成功地诱发了T细胞介导的特异性肝脏损害，其肝脏损害为ConA剂量依赖性；肝组织病理示，门管区大量淋巴细胞浸润，并可见点片状肝细胞坏死．以CSA预先抑制T细胞活化，则未见肝脏损害，肝组织病理也未发现淋巴细胞的浸润．结论ConA诱发的肝损害为无种属特异性的T细胞介导的肝损害，他为深入研究病毒性与自身免疫性肝损害的病理机制提供了又一方便理想的实验动物模型．

T cell dependent liver injury in Kunming mice induced by concanavalin A

AIM To establish T cell dependent liver injury model indued by concanavalin A (ConA) in Kunming mice.METHODS According to the Tiegs's work, ConA was administered to Kunming mice via tail vein.RESULTS The specific liver injury model induced by conA was dose depedent; The histoptbolooical examinations of liver specimen showed that T lymphocytes infiltratioo in portal areas, spot necrosis and piecemeal necrosis were seen. With the inhibition of T cells activation by cyclosporine A (CSA), liver injury and infiltration of lymphocytes were not seen.CONCLSION The Successful establishment of T cell dependent liver injury in Kunming mice initiated by ConA further proved that ConA induced liver injury is not restricted to a certain mouse strain, which also provides us a more easy and suitable liver injury model for studying the mechanisms of hepatasllular destruction of viral or autoimmune hepetitis.