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| 人NKG2D基因重组质粒的构建及鉴定 | |
| 引用本文： | 刘映霞,胡国龄,刘敏,何淑雅,龚环宇.人NKG2D基因重组质粒的构建及鉴定[J].南华大学学报(医学版),2003,31(4):369-372. |
| 作者姓名： | 刘映霞 胡国龄 刘敏 何淑雅 龚环宇 |
| 作者单位： | 1. 南华大学,第一附属医院,湖南,衡阳,421001 2. 中南大学,湘雅医院 3. 南华大学,生化教研室 |
| 基金项目： | 湖南省自然科学基金 ( 0 2JJY2 0 2 7)，省卫生厅基金 (Y0 2 -0 73 ) |
| 摘 要： | 目的 构建人NKG2D重组质粒。方法 应用RT-PCR，自健康人外周血单个核细胞(PB-MC)中获取NKG2D cDNA，克隆于pMDl8-T载体中，并经酶切和测序鉴定。结果 重组载体中插入片段序列与GeneBank登录的cDNA序列一致。结论 成功地构建了重组载体pMDl8T-NKG2D，为研究NKG2D的生物学活性奠定了基础。 |
| 关 键 词： | NKG2D 基因克隆 外周血单个核细胞 |
| 文章编号： | 1000-2510(2003)04-0369-04 |
| 修稿时间： | 2003年5月21日 |
Construction and Identification of Recombinant Plasmid of NKG2D Gene |
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| LIU Ying-xi,HU Guo-ling,LIU Min,et al.Construction and Identification of Recombinant Plasmid of NKG2D Gene[J].Journal of Nanhua University(Medical Edition),2003,31(4):369-372. | |
| Authors: | LIU Ying-xi HU Guo-ling LIU Min |
| Abstract: | Objective To construct recombinant vector of NKG2D gene. Methods NKG2D cDNA was obtained from peripheral blood mononuclear cells of human by using RT-PCR and was cloned into pMD18-T. The recombinant vector was identified with double restriction enzymes digestion and DNA sequence analysis. Results The sequence of NKG2D cDNA obtained from the recombinant vector was identical with that published on GeneBank. Conclusion The recombinant vector pMD18-NKG2D was constructed successfully,which lay the foundation for further studying biological activity of NKG2D. |
| Keywords: | NKG2D gene clone peripheral blood mononuclear cell |
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