息敏胶囊对IgE产生的实验研究

目的　探讨息敏胶囊抗Ⅰ型超敏反应的免疫学机制。方法　将Wistar大鼠随机分为 5组 :正常对照组 ,Ⅰ型超敏反应模型组 ,小剂量息敏胶囊组 ,大剂量息敏胶囊组 ,阳性药物对照组。以OVA腹腔注射免疫大鼠 ,采取各组动物抗血清 ,分别注入正常大鼠皮内 ,4 8h后以 5 %OVA 伊文氏蓝尾静脉注射 ,观察各组抗血清对正常大鼠被动皮肤过敏反应 (PCA)。结果　皮内注射小剂量息敏胶囊组、大剂量息敏胶囊组大鼠抗血清的动物注射部位皮片浸液吸光度明显低于皮内注射模型组大鼠抗血清的动物皮片浸液 (分别为P <0 .0 1,P <0 .0 1)。结论　息敏胶囊能明显减少OVA致敏大鼠IgE抗体的产生 ,从而抑制Ⅰ型超敏反应。

The experimental study of XiMin capsules on IgE production

Objective To study the immunological mechanism of XiMin capsules in antagonizing type Ⅰ allergy.Methods The Wistar rats were divided randomly into normal control group, type Ⅰ allergy model group, low-dosage of XiMin capsule group, high-dosage of XiMin capsule group and positive medicine group. Rats were sensitized by intraperitoneal injection(i.p) with OVA. Antisera from the animals of every group were harvested and respectively injected intracutaneously into other 10 normal rats, 48h later,the rats were injected intravenously in tail with 5% OVA -Even's Blue solution. The passive cutaneous anaphylaxis (PCA) was examined.Results The absorption (OD610)value of skin graft infusion of rats injected sera from the animals in low-dosage of XiMin capsule group and high-dosage of XiMin capsule group was significantly lower than that of rats injected sera from type Ⅰ allergy model group (P<0.01). Conclusion XiMin capsules might decrease the production of IgE and therefore inhibit type Ⅰ allergy.