| 紫草素对口腔鳞癌Tca8113细胞增殖与凋亡的作用 |
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| 引用本文： | 阮敏,杨雯君,周晓健,陈万涛,张陈平.紫草素对口腔鳞癌Tca8113细胞增殖与凋亡的作用[J].中国口腔颌面外科杂志,2010,8(1):66-72. |
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| 作者姓名： | 阮敏 杨雯君 周晓健 陈万涛 张陈平 |
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| 作者单位： | 上海交通大学医学院附属第九人民医院口腔医学院,口腔颌面外科,上海市口腔医学重点实验室,上海,200011 |
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| 基金项目： | 上海高校选拔培养优秀青年教师科研专项基金(jdy-08066)~~ |
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| 摘 要： | 目的：研究紫草素对体外培养的口腔鳞癌Tca8113细胞的增殖抑制及诱导凋亡作用。方法：采用四甲基偶氮唑蓝（MTT）法观察紫草素对Tca8113细胞的体外增殖抑制作用；采用光镜、透射电镜、琼脂糖凝胶电泳技术及流式细胞术观察紫草素对Tca8113细胞凋亡的影响。采用SPSS12．0软件包对数据进行单因素方差分析及t检验。结果：MTr检测显示．紫草素在0-50μmol／L浓度范围内。对Tca8113细胞的增殖抑制作用呈现时间依赖性和浓度依赖性（P〈0．01）．电镜下可见典型的细胞核皱缩及凋亡小体，DNA琼脂糖凝胶电泳观察到典型梯状条带，流式细胞仪结果显示亚G1期细胞明显增加，各组细胞凋亡率均显著高于对照组（P〈0．01）。结论：紫草素对口腔鳞癌Tca8113细胞具有明显的增殖抑制及诱导凋亡作用．可用于口腔鳞癌化学防治的新尝试。
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| 关 键 词： | 紫草素 口腔鳞癌 增殖抑制 细胞凋亡 |
Effect of Shikonin on human oral squamous cell carcinoma Tca8113 cell proliferation and apoptosis |
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| RUAN Min,YANG Wen-jun,ZHOU Xiao-jian,CHEN Wan-tao,ZHANG Chen-ping.Effect of Shikonin on human oral squamous cell carcinoma Tca8113 cell proliferation and apoptosis[J].China Journal of Oral and Maxillofacial Surgery,2010,8(1):66-72. |
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| Authors: | RUAN Min YANG Wen-jun ZHOU Xiao-jian CHEN Wan-tao ZHANG Chen-ping |
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| Institution: | RUAN Min,YANG Wen-jun,ZHOU Xiao-jian,CHEN Wan-tao,ZHANG Chen-ping.(Department of Oral , Maxillofacial Surgery,College of Stomatology,Ninth People\'s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai Key Laboratory of Stomatology.Shanghai 200011,China) |
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| Abstract: | PURPOSE:To investigate the effects of Shikonin on the proliferation and apoptosis of human oral squamous cell carcinoma Tca8113 cell line in vitro.METHODS:Inhibitory effect of Shikonin was determined by MTT assay,the morphological changes of the cancer cells were examined under light and electron microscopes,the DNA ladder and Flow cytometric analysis was performed to detect the cell cycle distribution and apoptosis.The data was analyzed by one-way ANOVA test and t test using SPSS12.0 software package.RESUL... |
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| Keywords: | Shikonin Oral squamous cell carcinoma(OSCC) Growth inhibition Apoptosis |
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