IL-32在慢性鼻窦炎组织中的表达及机理研究

目的探讨白介素-32（interleukin-32,IL-32）在慢性鼻窦炎组织中的表达及其致病机理。方法收集40例慢性鼻窦炎患者,同期选取20例行鼻部手术患者的正常组织作为对照者,采用酶联免疫吸附（ELISA）法检测两组鼻组织中IL-32蛋白;采取即用型EnVisionTMSuper/HRP检测试剂盒检测两组鼻黏膜组织中CD3＋和CD68＋表达。结果慢性鼻窦炎鼻黏膜组织中IL-32表达高于对照组（P〈0.01）,慢性鼻窦炎鼻黏膜组织中CD3＋细胞计数（55.36±23.53）个/mm2显著高于正常对照组（28.27±11.43）个/mm2（P〈0.01）;慢性鼻窦炎鼻黏膜组织中CD68＋细胞计数（7.65±2.27）个/mm2高于对照组（5.98±2.10）个/mm2（t=2.741,P〈0.01）。且慢性鼻窦炎鼻黏膜组织中IL-32与CD3＋和CD68＋表达呈正相关（P〈0.05）。结论 IL-32可能通过招募炎症细胞,参与慢性鼻窦炎的发病。

Expression and mechanism of interleuldn-32 in chronic rhinosinusitis

Objective To inverstigate the expression, and mechanism of interleukin-32 （ IL-32 ） in chronicrhinosinusitis （ CRS）. Methods The nasal tissue of 40 patients with CRS and 20 control cases were collected, andthe expression of IL-32 in specimens was detected with ELISA and CD3＋ , CD68＋ with immunohistochemistry. Thecorrelation between the expression of IL-32 and CD3 ＋ , CD68 ＋ was analyzed by Pearson correlation. Results Theexpression of IL-32 in the tissue of CRS patients was significantly higher than that of control group （ P 〈 0.01 ） . Thecount of CD3＋cell （55.36± 23.53 ） and CD68＋ cell （7.65± 2.27） in CRS patients were significantly higherthan thosesss in the control group （28. 27 ± 11. 43, 5. 98 ± 2. 10） respectively （P 〈 0. 01 ）. There was astatistically significant correlation between the expression of IL-32 and CD3＋ , CD68＋ （ r = 0. 484, 0. 432,respectively, P 〈 0. 05 ）. Conclusion IL-32 may participate in the pathogenesis of CRS through recruitinginflammatory cells.