乌司他丁对脓毒症大鼠血管内皮细胞功能保护作用研究

摘要：目的探讨乌司他丁是否通过抑制核因子-xB（nuclearfactor-κB，NF-κB）通路保护脓毒症大鼠血管内皮细胞功能。方法45只Wistar大鼠随机分为假手术组、模型组、乌司他丁组各15只，模型组与乌司他丁组应用盲肠结扎穿孔法构建脓毒症大鼠模型；假手术组与模型组腹腔注射生理盐水，乌司他丁组腹腔注射乌司他丁20u／g，连用3d；72h后3组测定血清血管性假血友病因子、可溶性细胞间黏附分子、可溶性血栓渊节蛋白水平及胸腹主动脉组织NF-κBp65表达。结果乌司他丁组血清血管性假血友病因子、可溶性细胞间黏附分子、可溶性血栓渊节蛋白水平及主动脉组织NF-κBp65表达明显低于模型组（P〈0．01），高于假手术组（P〈0．01）。结论乌司他丁可能通过抑制NF-κB表达保护脓毒症大鼠血管内皮细胞功能。

Effect of ulinastatin on protecting vascular endothelial cells in sepsis rats

Objective To explore the effect of ulinastatin on protecting vascular endothelial cells by inhibiting the expression of nuclear factor-κB （NF- κB） in sepsis rats. Methods Forty five Wistar rats were randomly divided into three groups： sham operation group, model group and ulinastatin group, with 15 rats in each group. The sepsis rat models were established with cecal ligation and puncture. Sham operation group and model group were given normal saline, and ulinastatin group was given 20 u/g ulinastatin with peritoneal injection totally for 3 days. The levels of serum von Willebrand factor （vWF）, soluble intercellular adhesion molecule 1 （SICAM 1） and soluble thrombomodulin （sTM） as well as NF-κB in thoracic and abdominal aorta tissue were detected 72 hours after models were established. Results The levels of serum vWF, SICAM-1, sTM and NF-κB p65 in ulinastatin group were significantly higher than those in sham operation group （P〈0.01）, and lower than those in model group （P〈0.01）. Conclusion Ulinastatin may protect vascular endothelial cell by inhibiting the expression of NF-κB in sepsis rats.