白消安致小鼠精子再生模型的精子发生量化评价

目的:量化评价白消安二次腹腔注射制备的小鼠精子再生模型。方法:54只8～10周龄雄性昆明白小鼠随机分为对照组和两个模型组,每组18只。模型组小鼠分别以10mg/kg和15mg/kg白消安间隔24d二次腹腔注射建立精子再生模型,对照组小鼠以50%二甲基亚砜溶液10ml/kg间隔24d二次腹腔注射。采用Johns-en评分量化给药后3、4和8周时生精上皮精子发生,运用实时定量PCR技术检测这3个时期支持细胞GATA结合蛋白4(GATA-4)mRNA和胶质细胞源性神经营养因子(GDNF)mRNA表达水平。结果:对照组Johnsen评分在各时期无显著差异(P>0.05)。给药后3周和4周,两模型组Johnsen评分均显著低于对照组(P<0.01);给药后4周和8周,15mg/kg组Johnsen评分均低于10mg/kg组(P<0.05);给药后8周,15mg/kg组Johnsen评分仍显著低于对照组(P<0.05),而10mg/kg组和对照组间无显著差异(P>0.05)。各组各时期支持细胞GATA-4mRNA表达均无显著差异(P>0.05)。对照组各时期支持细胞GDNF mRNA表达无显著差异(P>0.05)。两模型组支持细胞GDNF mRNA表达在给药后3周均高于对照组,而在给药后4周均低于对照组(P<0.01);给药后8周,15mg/kg组支持细胞GDNF mRNA表达低于对照组(P<0.01),而10mg/kg组与对照组无显著差异(P>0.05)。在以上3个时期,15mg/kg组GDNF mRNA表达均低于10mg/kg组(P<0.01)。结论:10mg/kg白消安间隔24d二次腹腔注射是建立小鼠精子再生模型的适宜剂量,增大剂量可使支持细胞GDNF mRNA表达不足,导致精子发生不能完全恢复。

Murine model of busulfan-induced spermatogenesis regeneration: A quantitative evaluation

Objective:To quantitatively evaluate the murine model of spermatogenesis regeneration induced by two-dose busulfan injection.Methods:Fifty-four male mice were randomly divided into a control and two model groups of equal number,the former treated by two-dose intraperitoneal injection of 50% DMSO solution at 10 ml/kg,and the latter by that of busulfan at 10 mg/kg and 15 mg/kg respectively to establish spermatogenesis regeneration models,both at the interval of 24 days between the two doses.Spermatogenesis in...