肝炎患者血清IL-2、sIL-2R水平和病毒含量变化

目的分析各型肝炎患者血清中IL_2s、IL_2R以及与HBV DNA、HCV RNA含量的关系变化。方法肝炎患者血清IL_2采用ELISA法,sIL_2R采用双抗体夹心间接ELISA法,HBV DNA定量和HCV RNA定量均采用荧光定量PCR法。结果急性肝炎、慢性乙型肝炎、慢性丙型肝炎、乙型肝炎后肝硬化和重型乙型肝炎患者血清中IL_2均低于正常对照组(P<0.05)。sIL_2R水平均高于正常对照组(P<0.05),其中以急性肝炎尤为明显(P<0.01)。慢性乙型肝炎、慢性丙型肝炎血清的病毒含量与sIL_2R水平呈正相关(P<0.05),但两组之间的sIL_2R水平无相关性(P>0.05)。结论肝病患者血清IL_2s、IL_2R可作为机体感染肝炎病毒后免疫水平和判断肝细胞受损程度的参考指标。

The changes of the levels of IL-2, sIL-2R and quantities of hepatitis virus in various hepatic diseases

Objective To investigate the changes of the levels of IL-2,sIL-2R and quantities of hepatitis virus in various hepatic diseases.Methods Enzyme-linked immunosorbent assay(ELISA),double antibody enzyme-linked immunosorbent assay and fluorogenic quantitative PCR(FQ-PCR) were used to detect the levels of IL-2,sIL-2R,HBV DNA and HCV RNA concentration in patients infected with hepatitis virus respectively.Results Levels of sera IL-2 in groups with acute viral hepatitis(AVH),chronic hepatitis B(CHB),chronic hepatitis C(CHC),cirrhosis and severe hepatitis B was significantly lower than that of control(P<0.05).But levels of sera sIL-2R was significantly higher than that of controls(P<0.05),and the level of sera sIL-2R in AVH group is more protrusted than others groups(P<0.05).Levels of sera sIL-2R have a positive correlation with the concentration of HBV DNA in CHB and HCV RNA in CHC,but there was no correlation between two groups in the level of IL-2(P>0.05). Conclusions The sera IL-2 and sIL-2R is the important markers of evaluating status of immunity of organism and determining the hepatic cell damnified degrees of hepatitis patients.