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Palbinone from Paeonia suffruticosa Protects Hepatic Cells via Up‐regulation of Heme Oxygenase‐1 |
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| Authors: | Do Thi Ha Tran Thi Phuong Joonseok Oh KiHwan Bae Nguyen Duy Thuan MinKyun Na |
| Institution: | 1. National Institute of Medicinal Materials, , Hoankiem, Hanoi, Vietnam;2. College of Pharmacy, Chungnam National University, , Daejeon, 305‐764 Republic of Korea;3. College of Pharmacy, Chonnam University, , Gwangju, 500‐757 Republic of Korea;4. Department of Pharmacognosy and Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, , University, MS, 38677 USA;5. Vietnam Academy of Traditional Medicine, , Hadong, Hanoi, Vietnam |
| Abstract: | Paeonia suffruticosa has been traditionally employed for vitalizing blood circulation and alleviating liver and inflammatory diseases. The pathways by which palbinone (PB) isolated from P. suffruticosa mediates heme oxygenase‐1 (HO‐1) induction were investigated using the specific inhibitors for PI3K and mitogen activated protein kinases pathways. The effect of PB‐treatment on Nrf2 translocalization and HO‐1‐antioxidant response element (ARE) regulation was examined employing Western blot and luciferase assays. PB induced HO‐1 expression via the activation of Nrf2 in the hepatic cells, and ARE‐dependent genes were stimulated via the PB‐mediated Nrf2 activation. PB‐mediated HO‐1 expression could be involved with PI3K/Akt and ERK1/2 pathways. Our study suggests the mechanism by which PB induces HO‐1 expression in the hepatic cells. This might substantiate the traditional applications of P. suffruticosa for the treatment of oxidative stress‐related diseases including oxidant and inflammatory‐mediated vascular and liver diseases. Copyright © 2013 John Wiley & Sons, Ltd. |
| Keywords: | Paeonia suffruticosa palbinone heme oxygenase‐1 Nrf2 activation PI3K/Akt pathways ERK1/2 pathways |