| 人参皂苷单体Rb1对多药耐药细胞系K562／HHT细胞内柔红霉素浓度的影响 |
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| 引用本文： | 史曦凯,张翼军,等.人参皂苷单体Rb1对多药耐药细胞系K562／HHT细胞内柔红霉素浓度的影响[J].重庆医学,2001,30(6):507-509. |
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| 作者姓名： | 史曦凯 张翼军 |
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| 作者单位： | 第三军医大学附属西南医院血液科 重庆400038
(史曦凯，张翼军)，第三军医大学附属西南医院血液科 重庆400038(赵春竟) |
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| 基金项目： | 重庆市科委应用基础研究基金 (1 9980 3 2 ) |
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| 摘 要： | 目的：探讨中药钙离子通道拮抗剂人参皂甙单体Rb1对白血病多药耐药细胞系（K562／HHT）细胞内柔红霉素浓度的影响。方法：细胞内柔红霉素浓度荧光测定法、细胞P－GP表达常规APAAP测定法、mdrl基因表达RT－PCR测定法等实验方法，探讨Rb1的逆转机理。结果：Rb1作用后K562／HHT细胞内DNR浓度增加（P＜0．01）。结论：K562／HHT是一种以P－GP介导的多药耐药细胞系，检测显示K562／HHT P－GP表达阳性率100％，并有很强的mdrl基因表达，提示Rb1通过抑制PGP外排药物功能，提高细胞内药物浓度，是Rbl逆转耐药作用的主要机理。
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| 关 键 词： | 耐药逆转 钙离子通道拮抗剂 人参皂甙Rb1 细胞内柔红霉素浓度 肿瘤 |
| 文章编号： | 1000-7911(2001)06-0507-03 |
The effects of saponins Rb1 on the intracellular concentration of DNR in human leukemic multi-drug resistant cell K562/HHT in vitro. |
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| SHI Xi kai,ZHANG Yi jun,ZHAO Chun jing.The effects of saponins Rb1 on the intracellular concentration of DNR in human leukemic multi-drug resistant cell K562/HHT in vitro.[J].Chongqing Medical Journal,2001,30(6):507-509. |
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| Authors: | SHI Xi kai ZHANG Yi jun ZHAO Chun jing |
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| Abstract: | Objective To explore the effects of saponins Rb1 on the intracellular concentration of DNR in human leukemic multi-drug resistant cell K562/HHT in vitro.Methods Through measurement of intracellular concentration of DNR by fluorimetry,detection of cell P GP with routine APAAP assay and detetmination of the mdr1 gene expression with RT PCT to explore the reversing mechanism of Rb1.Results Rb1 could increase cellular concentration of DNR in K562/HHT(P<0.01).The positive rate of P GP expression in K562/HHT even reached 100%.The mdr1 gene expression in K562/HHT is also stronger.Conclusion The mechanism of reversing effects of Rb1 is to increase the intracellular concentration of DNR through inhibiting drug discharge by P GP. |
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| Keywords: | reversal of multi-drug resistance calcium channel blocker Saponins Rb1 the intracellular concentration of DNR |
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