血管内皮生长因子C及其受体3在非小细胞肺癌组织中的表达及意义

目的　探讨血管内皮生长因子C(VEGF C)和受体 (VEGFR) 3在人非小细胞肺癌(NSCLC)组织中的表达及其与微血管、微淋巴管形成、淋巴转移、预后之间的关系。方法　对 76例人NSCLC及相应癌旁组织行VEGF C、VEGFR 3及CD34免疫组织化学染色链霉素抗生物素蛋白 过氧化物酶 (SP)法检测 ,进行淋巴管密度计数、微血管密度 (MVD)计数 ,并结合临床和病理资料进行分析。结果　NSCLC中 ,VEGF C的表达与肺癌分化程度负相关 (P =0 0 0 9)。VEGF C和VEGFR 3的表达水平与淋巴结转移呈正相关 (分别P =0 0 0 8,P =0 0 13) ,与淋巴浸润呈正相关 (分别P =0 0 2 7,P =0 0 2 0 )。VEGF C的表达与VEGFR 3在肺癌细胞中的表达呈正相关 (P =0 0 0 9)。VEGF C与淋巴管密度 (P =0 0 0 6 )、MVD(P =0 0 4 6 )呈正相关。淋巴管密度与淋巴结转移 (P =0 0 10 )、淋巴浸润 (P =0 0 19)、TNM分期 (P =0 0 15 )呈正相关 ,MVD与血行转移 (P <0 0 0 1)、TNM分期 (P <0 0 0 1)呈正相关。VEGF C阳性表达与生存时间、5年生存率呈负相关 (P <0 0 0 1)。结论　NSCLC中 ,VEGF C通过自分泌方式作用于受体VEGFR 3,促进肺癌组织生长 ,抑制分化。VEGF C促使肺癌内淋巴管形成 ,促进肺癌淋巴结转移。VEGF C和VEGFR 3表达增高、淋巴管密度增加

Expression of vascular endothelial growth factor (VEGF) C and VEGF receptor 3 in non-small cell lung cancer

Objective To study the relationship between angiogenesis and lymphangiogenesis with the expression of vascular endothelial growth factor C (VEGF C) and VEGFR 3 in human non small cell lung cancer (NSCLC) Methods Samples of 76 NSCLC cases with the neighboring noncancerous tissue were studied using anti VEGF C, VEGFR 3 and CD34 antibodies Assessment of lymphatic vessel density and microvessel density (MVD) were performed Results VEGF C expression in NSCLC was associating with the differentiation of tumor cells ( P =0 009) Expression of VEGF C and VEGFR 3 was significantly associated with lymph node metastasis ( P =0 008 and P =0 013 respectively) and lymphatic invasion ( P = 0 027 and P =0 020 respectively) A significant positive correlation was found between VEGF C in cancer cells and VEGFR 3 in lymphatic endothelial cells ( P =0 009) The number of lymphatic vessels ( P =0 006) and microvascular ( P =0 046) in VEGF C positive tumors was significantly larger than in VEGF C negative tumors Lymphatic vessel density was closely related to lymph node metastasis ( P =0 010), lymphatic invasion ( P =0.019) and clinical stages ( P =0.015) MVD was closely related to blood metastasis ( P <0 001) and clinical stages ( P <0.001) Patients with positive VEGF C expression had a worse prognosis than those with a negative VEGF C expression ( P <0 001) Conclusions VEGF C/VEGF D in NSCLCs, are related to lymphangiogenesis and angiogenesis, as well as to the occurrence and the development of lung cancers VEGF C promotes intratumoral lymphangiogenesis via VEGFR 3, resulting facilitated invasion of cancer cells into the lymphatic vessels VEGF C expression can be a useful predictor of poor prognosis in NSCLC