RNAi敲低小鼠睾丸雄激素结合蛋白SHBG对生精功能影响的研究

目的通过干扰小鼠睾丸雄激素结合蛋白(Sex hormone-binding globulin,SHBG)的表达,研究Shbg基因对雄性小鼠生精功能的影响。方法构建有效的Shbg基因干扰质粒,以Western Blot检测干扰效果,通过RNAi干扰小鼠睾丸Shbg基因的表达,研究干扰Shbg基因的表达对小鼠体内胎仔数和精子数量、精子活力、睾丸重量和体外受精率的影响。结果 RNAi干扰Shbg-mRNA的表达能使小鼠睾丸SHBG蛋白表达明显下调,SHBG蛋白表达下调使雄性小鼠自然交配后胎仔数降低,附睾精子的数量和体外受精率也明显下降。结论SHBG蛋白与雄性小鼠的生育力密切相关。

The study about the influence of testis SHBG on the spermatogenesis in male mice by attenuating the expression of Shbg with RNAi

Objective To study the influence of sex hormone- binding globulin （SHBG） in the testis on the spermatogenesis in male mice by attenuating the expression of gene Shbg with RNAi. Methods Constructed an effective gene Shbg interference plasmid RNAi and detected by western blot. RNAi technology was applied to interfere the expression of gene Shbg in testis of mice sperm count, sperm motility, testis weight and in vitro fertilization （IVF） rate and litter size were used to estimate the spermatogenesis in male mice. Results The RNAi plasmid targeting gene Shbg attenuated the expression of SHBG protein in testis of mice, reduced the fertility of male mice in vivo and in vitro including litter sizes and sperm density sperm, fertilizing capacity in vitro. Conclusion Testis SHBG protein,gene Shbg is related to the function of production in male mice and probably influenced the spermatogenesis and in vitro fertilization.