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| 复方大青叶注射液对体外培养HT-H9细胞CXCR4启动子活性的影响 | |
| 引用本文： | 冷弘,刘慧涛,臧玉翠,马云云,李敏,靳静,赵国强.复方大青叶注射液对体外培养HT-H9细胞CXCR4启动子活性的影响[J].郑州大学学报(医学版),2011,46(5):681-684. |
| 作者姓名： | 冷弘 刘慧涛 臧玉翠 马云云 李敏 靳静 赵国强 |
| 作者单位： | 1. 洛阳职业技术学院免疫学与病原生物学教研室 洛阳471000 2. 南阳油田总医院消化肾病科 南阳473132 3. 郑州大学基础医学院微生物学与免疫学教研室 郑州450001 4. 河南省职工医学院微生物学与免疫学教研室 郑州451191 |
| 基金项目： | 河南省医学科技攻关基金资助项目2008002002 |
| 摘 要： | 目的:探讨复方大青叶注射液对体外培养HT-H9细胞中CXCR4启动子活性的作用.方法:克隆人CXCR4启动子序列,构建报告载体pGL4.17-CXCR4;转染HT-H9细胞株,G418筛选后分组,高、低剂量实验组分别用200、100 mg/L大青液注射液处理,设注射水对照组、空白对照组和未转染对照组;各组处理24 h后... |
| 关 键 词： | 复方大青叶注射液 CXCR4启动子 艾滋病 HT-H9细胞 |
Effect of compound Folium Isatidis injection on activity of CXCR4 promoters in HT-H9 cells in vitro |
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| LENG Hong,LIU Huitao,ZANG Yucui,MA Yunyun,LI Min,JIN Jing,ZHAO Guoqiang.Effect of compound Folium Isatidis injection on activity of CXCR4 promoters in HT-H9 cells in vitro[J].Journal of Zhengzhou University: Med Sci,2011,46(5):681-684. | |
| Authors: | LENG Hong LIU Huitao ZANG Yucui MA Yunyun LI Min JIN Jing ZHAO Guoqiang |
| Institution: | LENG Hong1),LIU Huitao2),ZANG Yucui3),MA Yunyun4),LI Min3),JIN Jing4),ZHAO Guoqiang3) 1)Department of Immunology and Pathogen Biology,Luoyang Vocation & Technical College,Luoyang 471000 2)Department of Gastroenterology and Nephrology,Nanyang Oilfield General Hospital,Nanyang 473132 3)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 4)Department of Microbiology and Immunology,Henan Medical College for Staff and Workers,Zhengzhou 451191 |
| Abstract: | Aim:To investigate the effect of the compound Folium Isatidis injection on the activity of CXCR4 promoters in HT-H9 cells cultivated in vitro.Methods:The sequence of CXCR4 promoter gene was cloned,and inserted into the reporter vector pGL4.17 to construct luciferase reporter vector (pGL4.17-CXCR4),the recombinant plasmids were transfected into HT-H9 cells.Screened by G418,the lasting transfected cells were allocated into 4 groups: high dose group and low dose group (treated with 200,100 mg/L compound Folium... |
| Keywords: | compound Folium Isatidis injections CXCR4 promotor AIDS HT-H9 cells |
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