五味子乙素对HepG2细胞增殖、凋亡及内化阿霉素效应的影响

目的 分析不同浓度五味子乙素(Sch B)对肝癌细胞(HepG2)凋亡、增殖及内化阿霉素效应的影响.方法 体外培养HepG2细胞,分别加Sch B至不同浓度.采用流式细胞术分析Sch B作用24 h细胞的凋亡率;采用MTT还原实验检测该药物作用72 h增殖抑制率.阿霉素单独(20 μmol/L)或阿霉素与不同浓度的Sch B(0、25、50、75、100和150 μmol/L)共同处理4h,流式细胞术分析HepG2细胞内阿霉素分布.结果 SchB抑制HepG2增殖的IC50值为51.50μmol/L;但其浓度达100 μmol/L时,细胞发生凋亡,细胞凋亡率为(5.56±1.14)％.20 μmol/L阿霉素单独作用组荧光强度为58.30±3.93,而25 μmol/L Sch B同时作用组,荧光强度增加到88.22±4.85;随Seh B浓度增加细胞内荧光逐渐增强.结论 低浓度的Sch B已具有促进阿霉素内化的效应,但其抑制细胞增殖和诱导细胞凋亡的有效浓度则约为此浓度的2倍或4倍.

Analyzing the promotion of internalization of adriamycin by schisandrin B through flow cytometry

Objective To study the effect of schisandrin(Sch B)on HepG2 cells,including proliferation inhibition,apoptosis induction and the ability to promoting internalization of another medicine,adriamycin into cells.Methods Human liver cancer cells HepG2 were cultured in vitro in the presence of Sch B(0,25,50,100,150 and 200 μmol/L).The proliferation inhibition after treating for 72 hours and apoptosis induction for 24 hours were determined by-MTT reduction assay and flow cytomery(FCM) respectively.HepG2 cells were treated by adriamycin(20 μmol/L) alone or with different concentration of Sch B.After 4 hours,the fluorescence illuminated by adriamycin was detected by FCM.ResultsC50 of Sch B on HepG2 cells was 51.50 μmol/L.Apoptosis of HepG2 cells was found when the concentration of Sch B was elevated to 100 μmol/L.The mean value of fluorescence in cells treated with adriamycin alone was 58.30±3.93.Lower concentration of Sch B(25 μmol/L)could promoted that to 88.22±4.85.It was confirmed that the promotion effect increased in dose-dependent manner.Conclusion We found that lower concentration of Sch B could promote the internalization of adriamycin into tumor cells.While the concentration for inhibiting proliferation or inducing apoptosis was increased to 2 or 4 fold.