MK-801在脊髓缺血中的神经保护作用

目的地佐环平(MK-801)对脊髓缺血中神经保护作用的研究。方法建立新西兰兔脊髓缺血模型,利用HE染色、原位末端转移酶标记技术(TUNEL)、免疫组化、逆转录反应系统(RT-PCR)等技术检测N-甲基-D-天门冬氨酸受体(N-methyl-D-aspartate receptor,NMDAR)、诱导型一氧化氮合酶(inducible Nitric Oxide Synthase,iNOS)、半胱氨酸天冬氨酸蛋白酶3(Caspase-3)的表达水平,观察不同剂量MK-801在脊髓缺血中的神经保护作用。结果对照组脊髓结构完全消失,低剂量组结构较完整,高剂量组缺血损害程度最轻,假手术组脊髓结构正常。NMDAR、iNOS、Caspase-3等蛋白在神经元中有明确表达。凋亡指数、NMDAR、iNOS、Caspase-3 mRNA表达水平在对照组最高,低剂量组、高剂量组,假手术组则逐渐降低,差别具有统计学意义(P<0.05)。结论 MK-801能抑制神经细胞凋亡,对脊髓缺血具有神经保护作用。

The neuroprotective effect of MK-801 on spinal cord ischemic injury

Objective To study the neuroprotective effect of MK-801 on spinal cord ischemic injury.Methods Establishing spinal cord ischemia model of New Zealand white rabbits.Using the HE staining,TUNEL method,immunohischemistry staining and RT-PCR to detect expression levels of NMDAR,iNOS,caspase-3 and observe the neuroprotective effect of different doses of MK-801on spinal cord ischemic injury.Results Normal structure of the spinal cord completely disappeared in the control group.Structure of low-dose group is better than the control group.Sham group showed normal spinal cord structure.Ischemic damage of high dose group ranged from the low-dose group to the sham group.NMDAR,iNOS Caspase-3 were mainly located in the cytoplasm.Apoptotic index is the highest in the control group and from low dose group,high dose group to sham group gradually decreased with statistical significance(P<0.05).NMDAR,iNOS,Caspase-3 mRNA levels gradually decreased with statistical significancance from control group,low dose group,high dose group to sham group(P<0.05).Conclusions MK-801can inhibit the neuronal apoptosis.It has the neuroprotective effect.